RRC ID |
208
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著者 |
Mitsui N, Fujii T, Miyahara M, Oka T, Kashiwagi A, Kashiwagi K, Hanada H, Urushitani H, Santo N, Tooi O, Iguchi T.
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タイトル |
Development of metamorphosis assay using Silurana tropicalis for the detection of thyroid system-disrupting chemicals.
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ジャーナル |
Ecotoxicol Environ Saf
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Abstract |
The West African clawed frog (Silurana tropicalis), which resembles the South African clawed frog (Xenopus laevis), but is somewhat smaller, has a diploid genome and a shorter generation time. Therefore, S. tropicalis has the potential for use as a new model in ecotoxicology. We demonstrated a S. tropicalis metamorphosis assay based on Xenopus Metamorphosis Assay (XEMA) using 1 microg/L thyroxine (T4) and 75 mg/L propylthiouracil (PTU). Tadpoles at developmental stages 48-50 were exposed to chemicals for 28 days and total body length, developmental stage, and hind limb length were recorded every 7 days. Significant differences in developmental stage and total body length were found for both T4 and PTU after 7-day exposure, which were similar to the results of the XEMA ring-test using the same chemicals. Moreover, in the present study, we measured hind limb length as a new endpoint of thyroid axis. Significant differences in the hind limb length were encountered in both T4 and PTU treatments after 7 days of exposure. These results suggest that S. tropicalis can be used in a XEMA-like protocol to detect agonist and antagonist effects of chemicals on the thyroid system. Hind limb length is also a suitable endpoint in such protocols. A new test protocol detecting both thyroid disruption and reproductive effects of chemicals using S. tropicalis should be established in the near future.
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巻・号 |
64(3)
|
ページ |
281-7
|
公開日 |
2006-7-1
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DOI |
10.1016/j.ecoenv.2005.07.007
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PII |
S0147-6513(05)00177-6
|
PMID |
16139362
|
MeSH |
Animals
Antithyroid Agents / pharmacology*
Anura* / physiology
Biological Assay / methods*
Female
Hindlimb / drug effects
Hindlimb / growth & development
Hormone Antagonists / pharmacology*
Larva / drug effects
Larva / growth & development
Male
Metamorphosis, Biological / drug effects*
Propylthiouracil / pharmacology
Thyroid Gland / drug effects*
Thyroid Gland / physiopathology
Thyroxine / pharmacology
|
IF |
4.872
|
引用数 |
15
|
WOS 分野
|
ENVIRONMENTAL SCIENCES
TOXICOLOGY
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リソース情報 |
ツメガエル・イモリ |
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