論文 - 詳細
| RRC ID | 20914 |
|---|---|
| 著者 | Ohnuma M, Kuroiwa T, Tanaka K. |
| タイトル | Optimization of cryopreservation conditions for the unicellular red alga Cyanidioschyzon merolae. |
| ジャーナル | J Gen Appl Microbiol |
| Abstract |
Cryopreservation is essential for maintaining stable stocks of organisms. We report the development of a method for cryopreservation of the unicellular red alga Cyanidioschyzon merolae, a model organism for the investigation of the basic architecture of photosynthetic eukaryotes. Glycerol, dimethyl sulfoxide and methanol were examined for their ability to protect the cell from cryoinjury and/or cytotoxicity. It was found that methanol was the most effective as a cryoprotectant for C. merolae. After the optimized setting of parameters such as working concentration of cryoprotectant and the period of slow cooling, cultures were supplemented with 5% (v/v) methanol and frozen by slow cooling using a passive-freezing unit, followed by plunging into liquid nitrogen. We found C. merolae cells retained greater than 80% viability for at least 83 days in storage. |
| 巻・号 | 57(3) |
| ページ | 137-43 |
| 公開日 | 2011-1-1 |
| DOI | 10.2323/jgam.57.137 |
| PII | JST.JSTAGE/jgam/57.137 |
| PMID | 21817825 |
| MeSH | Cryopreservation / methods* Cryoprotective Agents / metabolism Dimethyl Sulfoxide / metabolism Freezing Glycerol / metabolism Methanol / metabolism Rhodophyta / radiation effects* Time Factors |
| IF | 1.442 |
| 引用数 | 4 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 藻類 | NIES-1332 |