RRC ID 20927
著者 Wang CY, Chen WL, Wang SW.
タイトル Pdc1 functions in the assembly of P bodies in Schizosaccharomyces pombe.
ジャーナル Mol Cell Biol
Abstract P bodies are cytoplasmic RNA granules containing the Dcp1-Dcp2 decapping enzymes where mRNA decay can occur. Here, we describe the characterization of P bodies in the fission yeast Schizosaccharomyces pombe. Most information on the property and function of P bodies stems from studies in the distantly related budding yeast Saccharomyces cerevisiae, and Edc3 was identified as a scaffold protein required for P-body assembly. However, we found that, unlike in S. cerevisiae, fission yeast Edc3 was dispensable for P-body formation. Pdc1, a novel partner of the fission yeast decapping enzyme, with a limited similarity to plant Edc4/Varicose that is required for the assembly of P bodies, was identified (tandem affinity purification-matrix-assisted laser desorption ionization tandem mass spectrometry [TAP-MALDI MS/MS]). Pdc1 interacts with Dcp2 through its C terminus and contains a coiled-coil region for self-interaction to mediate P-body formation. In line with the model that Pdc1 cross-bridges different proteins, additional interactions can be demonstrated with components such as Edc3 and Ste13. Although Pdc1 is not required for the interaction between Dcp1 and Dcp2, our data suggest that Pdc1 acts as a functional homologue of Edc4, a third component of the decapping enzymes that is thought to be absent from fungi. Together, these results highlight the diverse P-body protein compositions between different species and might help to provide insight into their evolutionary paths.
巻・号 33(6)
ページ 1244-53
公開日 2013-3-1
DOI 10.1128/MCB.01583-12
PII MCB.01583-12
PMID 23319050
PMC PMC3592021
MeSH Cell Line, Tumor Cytoplasmic Granules / genetics* Cytoplasmic Granules / metabolism* HeLa Cells Humans RNA, Fungal / genetics Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics* Schizosaccharomyces pombe Proteins / metabolism*
IF 3.611
引用数 14
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
酵母 FY15228 FY14544