| RRC ID |
20935
|
| Author |
Myosho T, Otake H, Masuyama H, Matsuda M, Kuroki Y, Fujiyama A, Naruse K, Hamaguchi S, Sakaizumi M.
|
| Title |
Tracing the emergence of a novel sex-determining gene in medaka, Oryzias luzonensis.
|
| Journal |
Genetics
|
| Abstract |
Three sex-determining (SD) genes, SRY (mammals), Dmy (medaka), and DM-W (Xenopus laevis), have been identified to date in vertebrates. However, how and why a new sex-determining gene appears remains unknown, as do the switching mechanisms of the master sex-determining gene. Here, we used positional cloning to search for the sex-determining gene in Oryzias luzonensis and found that GsdfY (gonadal soma derived growth factor on the Y chromosome) has replaced Dmy as the master sex-determining gene in this species. We found that GsdfY showed high expression specifically in males during sex differentiation. Furthermore, the presence of a genomic fragment that included GsdfY converts XX individuals into fertile XX males. Luciferase assays demonstrated that the upstream sequence of GsdfY contributes to the male-specific high expression. Gsdf is downstream of Dmy in the sex-determining cascade of O. latipes, suggesting that emergence of the Dmy-independent Gsdf allele led to the appearance of this novel sex-determining gene in O. luzonensis.
|
| Volume |
191(1)
|
| Pages |
163-70
|
| Published |
2012-5-1
|
| DOI |
10.1534/genetics.111.137497
|
| PII |
genetics.111.137497
|
| PMID |
22367037
|
| PMC |
PMC3338257
|
| MeSH |
Amino Acid Sequence
Animals
Computational Biology
Female
Fertility / genetics
Fish Proteins / chemistry
Fish Proteins / genetics
Fish Proteins / metabolism
Gene Expression Regulation
Male
Molecular Sequence Data
Mutation
Oryzias / genetics*
Oryzias / physiology
Sex Characteristics
Sex Chromosomes / genetics
Sex Determination Processes / genetics*
|
| IF |
4.015
|
| Times Cited |
224
|
|
WOS Category
|
GENETICS & HEREDITY
|
| Resource |
| Medaka |
O. luzonensis (RS270)
d-rR
Hd-rR-Tg-GsdfY (TG1318) |
