RRC ID 20936
Author Takehana Y, Naruse K, Asada Y, Matsuda Y, Shin-I T, Kohara Y, Fujiyama A, Hamaguchi S, Sakaizumi M.
Title Molecular cloning and characterization of the repetitive DNA sequences that comprise the constitutive heterochromatin of the W chromosomes of medaka fishes.
Journal Chromosome Res
Abstract Among the medaka fishes of the genus Oryzias, most species have homomorphic sex chromosomes, while some species, such as Oryzias hubbsi and Oryzias javanicus, have heteromorphic ZW sex chromosomes. In this study, a novel family of repetitive sequence was molecularly cloned from O. hubbsi and characterized by chromosome in situ and filter hybridization, respectively. This repetitive element, which we designated as a BstNI family element, localized at heterochromatin regions on the W chromosome, as well as on two pairs of autosomes. Homologous sequences to this element were found only in O. javanicus, which is a sister species of O. hubbsi, suggesting that this repeated element originated in the common ancestor of these two species. However, the intensity of the hybridization signals was lower in O. javanicus than in O. hubbsi, and the chromosomal location of this element in O. javanicus was confined to heterochromatin regions on one pair of autosomes. Thus, we hypothesize that this repetitive element was extensively amplified in the O. hubbsi lineage, especially on its W chromosome, after the separation of the O. javanicus lineage. In addition, we also found the W chromosomal location of the 18S-28S ribosomal RNA genes in both O. hubbsi and O. javanicus. Our previous studies showed no linkage homology of the sex chromosomes in these species, indicating that the RNA genes were shared between W chromosomes of different origins. This situation may be explained by a translocation of the sex-determining region with the ribosomal RNA genes in either species or an independent accumulation of the RNA genes as a convergent process during W chromosome degeneration.
Volume 20(1)
Pages 71-81
Published 2012-1-1
DOI 10.1007/s10577-011-9259-7
PMID 22124859
MeSH Animals Chromosome Mapping Cloning, Molecular / methods* Female Genes, rRNA Heterochromatin / genetics* Heterochromatin / metabolism In Situ Hybridization, Fluorescence Male Molecular Sequence Data Oryzias / classification Oryzias / genetics* Oryzias / metabolism Phylogeny RNA, Ribosomal, 18S / genetics RNA, Ribosomal, 28S / genetics Repetitive Sequences, Nucleic Acid* Sex Chromosomes / genetics* Sex Chromosomes / metabolism Sex Determination Processes Species Specificity
IF 3.413
Times Cited 18
WOS Category GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Medaka O. hubbsi (RS273) O. javanicus(Penang) (RS831) O. celebensis O. curvinotus O. dancena O. luzonensis (RS270) O. marmoratus (RS280) O. mekongensis O. minutillus