| RRC ID |
20983
|
| Author |
Wang L, Lu Y, Guan H, Jiang D, Guan Y, Zhang X, Nakano H, Zhou Y, Zhang Y, Yang L, Li H.
|
| Title |
Tumor necrosis factor receptor-associated factor 5 is an essential mediator of ischemic brain infarction.
|
| Journal |
J Neurochem
|
| Abstract |
Tumor necrosis factor receptor-associated factor 5 (TRAF5) is an adaptor protein of the tumor necrosis factor (TNF) receptor superfamily and the interleukin-1 receptor/Toll-like receptor superfamily and plays important roles in regulating multiple signaling pathways. This study was conducted to investigate the role of TRAF5 in the context of brain ischemia/reperfusion (I/R) injury. Transient occlusion of the middle cerebral artery was performed on TRAF5 knockout mice (KO), neuron-specific TRAF5 transgene (TG), and the appropriate controls. Compared with the WT mice, the TRAF5 KO mice showed lower infarct volumes and better outcomes in the neurological tests. A low neuronal apoptosis level, an attenuated blood-brain barrier (BBB) disruption and an inhibited inflammatory response were exhibited in TRAF5 KO mice. TRAF5 TG mice exhibited an opposite phenotype. Moreover, the Akt/FoxO1 signaling pathway was enhanced in the ischemic brains of the TRAF5 KO mice. These results provide the first demonstration that TRAF5 is a critical mediator of I/R injury in an experimental stroke model. The Akt /FoxO1 signaling pathway probably plays an important role in the biological function of TRAF5 in this model.
|
| Volume |
126(3)
|
| Pages |
400-14
|
| Published |
2013-8-1
|
| DOI |
10.1111/jnc.12207
|
| PMID |
23413803
|
| MeSH |
Animals
Blotting, Western
Brain Infarction / metabolism*
Brain Infarction / pathology
Brain Ischemia / metabolism
Brain Ischemia / pathology
Disease Models, Animal
Fluorescent Antibody Technique
In Situ Nick-End Labeling
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Real-Time Polymerase Chain Reaction
Reperfusion Injury / metabolism
Signal Transduction / physiology*
TNF Receptor-Associated Factor 5 / metabolism*
|
| IF |
4.066
|
| Times Cited |
25
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
NEUROSCIENCES
|
| Resource |
| Mice |
RBRC02573 |
