RRC ID 21090
Author Shao J, Xu Y, Wang Z, Jiang Y, Yu G, Peng X, Li R.
Title Elucidating the toxicity targets of β-ionone on photosynthetic system of Microcystis aeruginosa NIES-843 (Cyanobacteria).
Journal Aquat. Toxicol.
Abstract In order to explore the potential targets of toxicity of β-ionone on the photosynthetic system of Microcystis aeruginosa, the polyphasic rise in chlorophyll a (Chl a) fluorescence transient and transcript expression for key genes in photosystem II (PSII) of M. aeruginosa NIES-843 were studied. The EC₅₀ value of β-ionone on M. aeruginosa NIES-843 was found to be 21.23±1.87 mg/L. It was shown that β-Ionone stress can lead to a decrease in pigment content of M. aeruginosa NIES-843 cells, and that carotenoids were more sensitive to β-ionone stress than Chl a. The normalized Chl a fluorescence transients were slightly decreased at 6.67 and 10 mg/L β-ionone, but significantly increased at 15, 22.5 and 33.75 mg/L. There was no significant variation on transcript expression of psbA and psbO at a concentration of 6.67 mg/L β-ionone, but they were down-regulated at 22.5 mg/L. Ultrastructural examination by transmission electron microscopy indicated that the thylakoids were distorted, and the thylakoid membrane stacks began to collapse when M. aeruginosa NIES-843 was exposed to β-ionone at a concentration of 22.5 and 33.75 mg/L. Our results indicate that the reaction centre of PS II and the electron transport at the acceptor side of PS II are the targets responsible for the toxicity of β-ionone on the PS II of M. aeruginosa NIES-843.
Volume 104(1-2)
Pages 48-55
Published 2011-7
DOI 10.1016/j.aquatox.2011.03.014
PII S0166-445X(11)00093-2
PMID 21543049
MeSH Chlorophyll / metabolism Chlorophyll A Gene Expression / drug effects Microcystis / drug effects* Microcystis / physiology Microcystis / ultrastructure Norisoprenoids / toxicity* Photosynthesis / drug effects* Photosynthesis / genetics Water Pollutants, Chemical / toxicity*
IF 3.794
Times Cited 31
Algae NIES-843