RRC ID |
21090
|
著者 |
Shao J, Xu Y, Wang Z, Jiang Y, Yu G, Peng X, Li R.
|
タイトル |
Elucidating the toxicity targets of β-ionone on photosynthetic system of Microcystis aeruginosa NIES-843 (Cyanobacteria).
|
ジャーナル |
Aquat Toxicol
|
Abstract |
In order to explore the potential targets of toxicity of β-ionone on the photosynthetic system of Microcystis aeruginosa, the polyphasic rise in chlorophyll a (Chl a) fluorescence transient and transcript expression for key genes in photosystem II (PSII) of M. aeruginosa NIES-843 were studied. The EC₅₀ value of β-ionone on M. aeruginosa NIES-843 was found to be 21.23±1.87 mg/L. It was shown that β-Ionone stress can lead to a decrease in pigment content of M. aeruginosa NIES-843 cells, and that carotenoids were more sensitive to β-ionone stress than Chl a. The normalized Chl a fluorescence transients were slightly decreased at 6.67 and 10 mg/L β-ionone, but significantly increased at 15, 22.5 and 33.75 mg/L. There was no significant variation on transcript expression of psbA and psbO at a concentration of 6.67 mg/L β-ionone, but they were down-regulated at 22.5 mg/L. Ultrastructural examination by transmission electron microscopy indicated that the thylakoids were distorted, and the thylakoid membrane stacks began to collapse when M. aeruginosa NIES-843 was exposed to β-ionone at a concentration of 22.5 and 33.75 mg/L. Our results indicate that the reaction centre of PS II and the electron transport at the acceptor side of PS II are the targets responsible for the toxicity of β-ionone on the PS II of M. aeruginosa NIES-843.
|
巻・号 |
104(1-2)
|
ページ |
48-55
|
公開日 |
2011-7-1
|
DOI |
10.1016/j.aquatox.2011.03.014
|
PII |
S0166-445X(11)00093-2
|
PMID |
21543049
|
MeSH |
Chlorophyll / metabolism
Chlorophyll A
Gene Expression / drug effects
Microcystis / drug effects*
Microcystis / physiology
Microcystis / ultrastructure
Norisoprenoids / toxicity*
Photosynthesis / drug effects*
Photosynthesis / genetics
Water Pollutants, Chemical / toxicity*
|
IF |
4.346
|
引用数 |
48
|
WOS 分野
|
MARINE & FRESHWATER BIOLOGY
TOXICOLOGY
|
リソース情報 |
藻類 |
NIES-843 |