| RRC ID |
21120
|
| Author |
Tanaka T, Matsumoto S, Yamada M, Yamada R, Matsuda F, Kondo A.
|
| Title |
Display of active beta-glucosidase on the surface of Schizosaccharomyces pombe cells using novel anchor proteins.
|
| Journal |
Appl Microbiol Biotechnol
|
| Abstract |
Here, we demonstrate display of beta-glucosidase (BGL) on the surface of Schizosaccharomyces pombe cells using novel anchor proteins. A total of four candidate anchor proteins (SPBC21D10.06c, SPBC947.04, SPBC19C7.05, and SPBC359.04c) were selected from among almost all of S. pombe membrane proteins. The C-terminus of each anchor protein was genetically fused to the N-terminus of BGL, and the fusion protein was expressed using S. pombe as a host. The highest cell surface-associated BGL activity (107 U/10(5) cells was achieved with SPBC359.04c serving as the anchor, followed by SPBC947.04 (44 U/10(5) cells) and SPBC21D10.06c (38 U/10(5) cells). S. pombe displaying BGL with SPBC359.04c as an anchor showed the highest growth on 2 % cellobiose (10.7 × 10(7) cells/mL after 41 h of cultivation from an initial density of 0.1 × 10(7) cells/mL). Additionally, culturing BGL-displaying S. pombe in medium containing cellobiose as the sole carbon source did not affect protein expression, and ethanol fermentation from cellobiose was successfully demonstrated using BGL-displaying S. pombe. This is the first report describing a cell surface display system for the functionalization of S. pombe.
|
| Volume |
97(10)
|
| Pages |
4343-52
|
| Published |
2013-5-1
|
| DOI |
10.1007/s00253-013-4733-0
|
| PMID |
23385477
|
| MeSH |
Base Sequence
Cellobiose / metabolism
DNA Primers
Fermentation
Molecular Sequence Data
Plasmids
Schizosaccharomyces / enzymology*
beta-Glucosidase / metabolism*
|
| IF |
3.53
|
| Times Cited |
5
|
|
WOS Category
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| Resource |
| DNA material |
pDUAL-FFH51 (RDB06537)
pDUAL2-HFG1 (RDB06196) |
| Yeast |
|
