RRC ID 21127
著者 Nakano T, Naito Y, Kakegawa K, Ohtsuki N, Tsujimoto-Inui Y, Shinshi H, Suzuki K.
タイトル Increase in pectin deposition by overexpression of an ERF gene in cultured cells of Arabidopsis thaliana.
ジャーナル Biotechnol Lett
Abstract Ethylene-responsive transcription factor (ERF) family genes, which are involved in regulation of metabolic pathways and/or are useful for metabolic engineering, were investigated in the cultured cells of Arabidopsis thaliana. The pectin content in the gelatinous precipitates after the ethanol precipitation of extracts derived from calli of a transgenic cell line, A17, overexpressing an ERF gene (At1g44830), increased in comparison with the control. Expression of genes involved in pectin biosynthesis was up-regulated in the A17 calli. Overexpression of the ERF gene coordinately activates the pectin biosynthetic pathway genes and increases the content of pectin. These results therefore will be useful as a genetic resource for engineering pectin biosynthesis in plants.
巻・号 34(4)
ページ 763-9
公開日 2012-4-1
DOI 10.1007/s10529-011-0826-y
PMID 22160296
MeSH Arabidopsis / genetics* Arabidopsis / metabolism* Biosynthetic Pathways / genetics Cells, Cultured DNA-Binding Proteins / biosynthesis* DNA-Binding Proteins / genetics Gene Expression* Pectins / biosynthesis* Plant Proteins / biosynthesis* Plant Proteins / genetics
IF 1.977
引用数 4
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 rpc00008