論文 - 詳細
| RRC ID | 21129 |
|---|---|
| 著者 | Verbitskiy D, Merwe JA, Zehrmann A, Härtel B, Takenaka M. |
| タイトル | The E-class PPR protein MEF3 of Arabidopsis thaliana can also function in mitochondrial RNA editing with an additional DYW domain. |
| ジャーナル | Plant Cell Physiol |
| Abstract |
In plants, RNA editing is observed in mitochondria and plastids, changing selected C nucleotides into Us in both organelles. We here identify the PPR (pentatricopeptide repeat) protein MEF3 (mitochondrial editing factor 3) of the E domain PPR subclass by genetic mapping of a variation between ecotypes Columbia (Col) and Landsberg erecta (Ler) in Arabidopsis thaliana to be required for a specific RNA editing event in mitochondria. The Ler variant of MEF3 differs from Col in two amino acids in repeats 9 and 10, which reduce RNA editing levels at site atp4-89 to about 50% in Ler. In a T-DNA insertion line, editing at this site is completely lost. In Vitis vinifera the gene most similar to MEF3 continues into a DYW extension beyond the common E domain. Complementation assays with various combinations of PPR and E domains from the vine and A. thaliana proteins show that the vine E region can substitute for the A. thaliana E region with or without the DYW domain. These findings suggest that the additional DYW domain does not disturb the MEF3 protein function in mitochondrial RNA editing in A. thaliana. |
| 巻・号 | 53(2) |
| ページ | 358-67 |
| 公開日 | 2012-2-1 |
| DOI | 10.1093/pcp/pcr182 |
| PII | pcr182 |
| PMID | 22186180 |
| MeSH | Arabidopsis / genetics* Arabidopsis / metabolism Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Base Sequence Chromosome Mapping DNA, Bacterial Ecotype Genetic Complementation Test Mitochondria / genetics Mitochondria / metabolism* Molecular Sequence Data Mutagenesis, Insertional Protein Interaction Domains and Motifs RNA Editing* RNA, Plant / metabolism* |
| IF | 4.062 |
| 引用数 | 21 |
| WOS 分野 | PLANT SCIENCES CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | psh12413 |