| RRC ID |
21139
|
| 著者 |
Chen MQ, Zhang AH, Zhang Q, Zhang BC, Nan J, Li X, Liu N, Qu H, Lu CM, Sudmorgen, Zhou YH, Xu ZH, Bai SN.
|
| タイトル |
Arabidopsis NMD3 is required for nuclear export of 60S ribosomal subunits and affects secondary cell wall thickening.
|
| ジャーナル |
PLoS One
|
| Abstract |
NMD3 is required for nuclear export of the 60S ribosomal subunit in yeast and vertebrate cells, but no corresponding function of NMD3 has been reported in plants. Here we report that Arabidopsis thaliana NMD3 (AtNMD3) showed a similar function in the nuclear export of the 60S ribosomal subunit. Interference with AtNMD3 function by overexpressing a truncated dominant negative form of the protein lacking the nuclear export signal sequence caused retainment of the 60S ribosomal subunits in the nuclei. More interestingly, the transgenic Arabidopsis with dominant negative interference of AtNMD3 function showed a striking failure of secondary cell wall thickening, consistent with the altered expression of related genes and composition of cell wall components. Observation of a significant decrease of rough endoplasmic reticulum (RER) in the differentiating interfascicular fiber cells of the transgenic plant stems suggested a link between the defective nuclear export of 60S ribosomal subunits and the abnormal formation of the secondary cell wall. These findings not only clarified the evolutionary conservation of NMD3 functions in the nuclear export of 60S ribosomal subunits in yeast, animals and plants, but also revealed a new facet of the regulatory mechanism underlying secondary cell wall thickening in Arabidopsis. This new facet is that the nuclear export of 60S ribosomal subunits and the formation of RER may play regulatory roles in coordinating protein synthesis in cytoplasm and transcription in nuclei.
|
| 巻・号 |
7(4)
|
| ページ |
e35904
|
| 公開日 |
2012-1-1
|
| DOI |
10.1371/journal.pone.0035904
|
| PII |
PONE-D-11-21452
|
| PMID |
22558264
|
| PMC |
PMC3338764
|
| MeSH |
Active Transport, Cell Nucleus / genetics
Arabidopsis / genetics*
Arabidopsis / metabolism
Arabidopsis Proteins / genetics*
Arabidopsis Proteins / metabolism
Cell Nucleus / metabolism
Cell Wall / genetics
Cell Wall / metabolism*
Cell Wall / ultrastructure
Cytoplasm / metabolism
Endoplasmic Reticulum / genetics
Endoplasmic Reticulum / metabolism
Gene Expression
Nuclear Export Signals / genetics
Nucleocytoplasmic Transport Proteins / genetics*
Nucleocytoplasmic Transport Proteins / metabolism
Phylogeny
Plants, Genetically Modified / genetics*
Plants, Genetically Modified / metabolism
Protein Biosynthesis
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Ribosome Subunits, Large, Eukaryotic / genetics*
Ribosome Subunits, Large, Eukaryotic / metabolism
Transformation, Genetic
|
| IF |
2.74
|
| 引用数 |
11
|
|
WOS 分野
|
PLANT SCIENCES
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
pst14457 |
