RRC ID 21146
著者 Pandey RP, Malla S, Simkhada D, Kim BG, Sohng JK.
タイトル Production of 3-O-xylosyl quercetin in Escherichia coli.
ジャーナル Appl Microbiol Biotechnol
Abstract Quercetin, a flavonol aglycone, is one of the most abundant flavonoids with high medicinal value. The bioavailability and pharmacokinetic properties of quercetin are influenced by the type of sugars attached to the molecule. To efficiently diversify the therapeutic uses of quercetin, Escherichia coli was harnessed as a production factory by the installation of various plant and bacterial UDP-xylose sugar biosynthetic genes. The genes encoding for the UDP-xylose pathway enzymes phosphoglucomutase (nfa44530), glucose-1-phosphate uridylyltransferase (galU), UDP-glucose dehydrogenase (calS8), and UDP-glucuronic acid decarboxylase (calS9) were overexpressed in E. coli BL21 (DE3) along with a glycosyltransferase (arGt-3) from Arabidopsis thaliana. Furthermore, E. coli BL21(DE3)/∆pgi, E. coli BL21(DE3)/∆zwf, E. coli BL21(DE3)/∆pgi∆zwf, and E. coli BL21(DE3)/∆pgi∆zwf∆ushA mutants carrying the aforementioned UDP-xylose sugar biosynthetic genes and glycosyltransferase and the galU-integrated E. coli BL21(DE3)/∆pgi host harboring only calS8, calS9, and arGt-3 were constructed to enhance whole-cell bioconversion of exogeneously supplied quercetin into 3-O-xylosyl quercetin. Here, we report the highest production of 3-O-xylosyl quercetin with E. coli BL21 (DE3)/∆pgi∆zwf∆ushA carrying UDP-xylose sugar biosynthetic genes and glycosyltransferase. The maximum concentration of 3-O-xylosyl quercetin achieved was 23.78 mg/L (54.75 μM), representing 54.75 % bioconversion, which was an ~4.8-fold higher bioconversion than that shown by E. coli BL21 (DE3) with the same set of genes when the reaction was carried out in 5-mL culture tubes with 100 μM quercetin under optimized conditions. Bioconversion was further improved by 98 % when the reaction was scaled up in a 3-L fermentor at 36 h.
巻・号 97(5)
ページ 1889-901
公開日 2013-3-1
DOI 10.1007/s00253-012-4438-9
PMID 23053089
MeSH Biotransformation Cloning, Molecular Escherichia coli / genetics Escherichia coli / metabolism* Gene Expression Metabolic Engineering* Metabolic Networks and Pathways / genetics Plant Proteins / genetics Plant Proteins / metabolism Quercetin / analogs & derivatives* Quercetin / biosynthesis* Recombinant Proteins / genetics Recombinant Proteins / metabolism
IF 3.53
引用数 65
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda09852 pda01903 pda05007 pda02106 pda01013 pda13073 pda18540