論文 - 詳細
| RRC ID | 21154 |
|---|---|
| 著者 | Norman-Axelsson U, Durand-Dubief M, Prasad P, Ekwall K. |
| タイトル | DNA topoisomerase III localizes to centromeres and affects centromeric CENP-A levels in fission yeast. |
| ジャーナル | PLoS Genet |
| Abstract |
Centromeres are specialized chromatin regions marked by the presence of nucleosomes containing the centromere-specific histone H3 variant CENP-A, which is essential for chromosome segregation. Assembly and disassembly of nucleosomes is intimately linked to DNA topology, and DNA topoisomerases have previously been implicated in the dynamics of canonical H3 nucleosomes. Here we show that Schizosaccharomyces pombe Top3 and its partner Rqh1 are involved in controlling the levels of CENP-A(Cnp1) at centromeres. Both top3 and rqh1 mutants display defects in chromosome segregation. Using chromatin immunoprecipitation and tiling microarrays, we show that Top3, unlike Top1 and Top2, is highly enriched at centromeric central domains, demonstrating that Top3 is the major topoisomerase in this region. Moreover, centromeric Top3 occupancy positively correlates with CENP-A(Cnp1) occupancy. Intriguingly, both top3 and rqh1 mutants display increased relative enrichment of CENP-A(Cnp1) at centromeric central domains. Thus, Top3 and Rqh1 normally limit the levels of CENP-A(Cnp1) in this region. This new role is independent of the established function of Top3 and Rqh1 in homologous recombination downstream of Rad51. Therefore, we hypothesize that the Top3-Rqh1 complex has an important role in controlling centromere DNA topology, which in turn affects the dynamics of CENP-A(Cnp1) nucleosomes. |
| 巻・号 | 9(3) |
| ページ | e1003371 |
| 公開日 | 2013-1-1 |
| DOI | 10.1371/journal.pgen.1003371 |
| PII | PGENETICS-D-12-01254 |
| 解説 | top3-105 rad51Δ |
| PMID | 23516381 |
| PMC | PMC3597498 |
| MeSH | Centromere* / genetics Centromere* / ultrastructure Chromatin / genetics Chromatin / ultrastructure Chromosomal Proteins, Non-Histone* / genetics Chromosomal Proteins, Non-Histone* / metabolism Chromosome Segregation / genetics DNA Helicases* / genetics DNA Helicases* / metabolism DNA Topoisomerases, Type I* / genetics DNA Topoisomerases, Type I* / metabolism Histones / genetics Histones / metabolism Homologous Recombination Kinetochores / ultrastructure Nucleosomes / genetics Rad51 Recombinase / genetics Schizosaccharomyces* / genetics Schizosaccharomyces* / metabolism Schizosaccharomyces pombe Proteins* / genetics Schizosaccharomyces pombe Proteins* / metabolism |
| IF | 5.175 |
| 引用数 | 6 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | FY8062 |