RRC ID 21154
Author Norman-Axelsson U, Durand-Dubief M, Prasad P, Ekwall K.
Title DNA topoisomerase III localizes to centromeres and affects centromeric CENP-A levels in fission yeast.
Journal PLoS Genet.
Abstract Centromeres are specialized chromatin regions marked by the presence of nucleosomes containing the centromere-specific histone H3 variant CENP-A, which is essential for chromosome segregation. Assembly and disassembly of nucleosomes is intimately linked to DNA topology, and DNA topoisomerases have previously been implicated in the dynamics of canonical H3 nucleosomes. Here we show that Schizosaccharomyces pombe Top3 and its partner Rqh1 are involved in controlling the levels of CENP-A(Cnp1) at centromeres. Both top3 and rqh1 mutants display defects in chromosome segregation. Using chromatin immunoprecipitation and tiling microarrays, we show that Top3, unlike Top1 and Top2, is highly enriched at centromeric central domains, demonstrating that Top3 is the major topoisomerase in this region. Moreover, centromeric Top3 occupancy positively correlates with CENP-A(Cnp1) occupancy. Intriguingly, both top3 and rqh1 mutants display increased relative enrichment of CENP-A(Cnp1) at centromeric central domains. Thus, Top3 and Rqh1 normally limit the levels of CENP-A(Cnp1) in this region. This new role is independent of the established function of Top3 and Rqh1 in homologous recombination downstream of Rad51. Therefore, we hypothesize that the Top3-Rqh1 complex has an important role in controlling centromere DNA topology, which in turn affects the dynamics of CENP-A(Cnp1) nucleosomes.
Volume 9(3)
Pages e1003371
Published 2013
DOI 10.1371/journal.pgen.1003371
PII PGENETICS-D-12-01254
Description top3-105 rad51Δ
PMID 23516381
PMC PMC3597498
MeSH Centromere* / genetics Centromere* / ultrastructure Chromatin / genetics Chromatin / ultrastructure Chromosomal Proteins, Non-Histone* / genetics Chromosomal Proteins, Non-Histone* / metabolism Chromosome Segregation / genetics DNA Helicases* / genetics DNA Helicases* / metabolism DNA Topoisomerases, Type I* / genetics DNA Topoisomerases, Type I* / metabolism Histones / genetics Histones / metabolism Homologous Recombination Kinetochores / ultrastructure Nucleosomes / genetics Rad51 Recombinase / genetics Schizosaccharomyces* / genetics Schizosaccharomyces* / metabolism Schizosaccharomyces pombe Proteins* / genetics Schizosaccharomyces pombe Proteins* / metabolism
IF 5.54
Times Cited 4
WOS Category GENETICS & HEREDITY
Resource
Yeast FY8062