| RRC ID |
21306
|
| Author |
Iino T, Suzuki R, Tanaka N, Kosako Y, Ohkuma M, Komagata K, Uchimura T.
|
| Title |
Gluconacetobacter kakiaceti sp. nov., an acetic acid bacterium isolated from a traditional Japanese fruit vinegar.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
Two novel acetic acid bacteria, strains G5-1(T) and I5-1, were isolated from traditional kaki vinegar (produced from fruits of kaki, Diospyros kaki Thunb.), collected in Kumamoto Prefecture, Japan. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains G5-1(T) and I5-1 formed a distinct subline in the genus Gluconacetobacter and were closely related to Gluconacetobacter swingsii DST GL01(T) (99.3% 16S rRNA gene sequence similarity). The isolates showed 96-100% DNA-DNA relatedness with each other, but <53% DNA-DNA relatedness with closely related members of the genus Gluconacetobacter. The isolates could be distinguished from closely related members of the genus Gluconacetobacter by not producing 2- and 5-ketogluconic acids from glucose, producing cellulose, growing without acetic acid and with 30% (w/v) d-glucose, and producing acid from sugars and alcohols. Furthermore, the genomic DNA G+C contents of strains G5-1(T) and I5-1 were a little higher than those of their closest phylogenetic neighbours. On the basis of the phenotypic characteristics and phylogenetic position, strains G5-1(T) and I5-1 are assigned to a novel species, for which the name Gluconacetobacter kakiaceti sp. nov. is proposed; the type strain is G5-1(T) (=JCM 25156(T)=NRIC 0798(T)=LMG 26206(T)).
|
| Volume |
62(Pt 7)
|
| Pages |
1465-1469
|
| Published |
2012-7-1
|
| DOI |
10.1099/ijs.0.031773-0
|
| PMID |
21841006
|
| MeSH |
Acetic Acid / metabolism*
Bacterial Typing Techniques
Base Composition
Cluster Analysis
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Food Microbiology*
Fruit
Gluconacetobacter / classification*
Gluconacetobacter / genetics
Gluconacetobacter / isolation & purification*
Japan
Molecular Sequence Data
Nucleic Acid Hybridization
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
|
| IF |
2.415
|
| Times Cited |
14
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| General Microbes |
JCM 7644
JCM 9730
JCM 16935
JCM 16936
JCM 16937
JCM 17122
JCM 17123
JCM 17840
JCM 25120
JCM 25121
JCM 25156
JCM 25160 |
