| RRC ID |
21310
|
| 著者 |
Vajna B, Kanizsai S, Kéki Z, Márialigeti K, Schumann P, Tóth EM.
|
| タイトル |
Thermus composti sp. nov., isolated from oyster mushroom compost.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
A Gram-stain-negative, rod-shaped bacterium (strain K-39(T)) was isolated from the thermophilic phase of the composting process for oyster mushroom substrate preparation. The strain grew at 40-80 °C (optimum, 65-75 °C), at pH 5-9 (optimum, pH 7), in media containing up to 1.5% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain K-39(T) formed a distinct lineage within the genus Thermus. Its closest cultivated relative was Thermus islandicus PRI 3838(T) (96.8% similarity). The DNA G+C content of strain K-39(T) was 71.3 mol%. The new strain could be differentiated from the related taxa by not being able to hydrolyse starch. The predominant fatty acids of strain K-39(T) were iso-C(17:0) and anteiso-C(17:0). Strain K-39(T) contained a lower amount of the fatty acid iso-C(15:0) as compared to related species of the genus Thermus. The predominant respiratory quinone of the new isolate was menaquinone MK-8. On the basis of a taxonomic study using a polyphasic approach, strain K-39(T) is considered to represent a novel species of the genus Thermus, for which the name Thermus composti sp. nov. is proposed. The type strain is K-39(T) (=DSM 21686(T)=NCAIM B 02340(T)).
|
| 巻・号 |
62(Pt 7)
|
| ページ |
1486-1490
|
| 公開日 |
2012-7-1
|
| DOI |
10.1099/ijs.0.030866-0
|
| PMID |
21856987
|
| MeSH |
Bacterial Typing Techniques
Base Composition
Cluster Analysis
Culture Media / chemistry
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Fatty Acids / analysis
Hydrogen-Ion Concentration
Molecular Sequence Data
Phylogeny
Pleurotus*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Sodium Chloride / metabolism
Soil Microbiology*
Temperature
Thermus / classification*
Thermus / genetics
Thermus / isolation & purification*
Thermus / physiology
Vitamin K 2 / analysis
|
| IF |
2.415
|
| 引用数 |
16
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 15153 |
