RRC ID 21324
著者 Liu C, Chen CX, Zhang XY, Yu Y, Liu A, Li GW, Chen XL, Chen B, Zhou BC, Zhang YZ.
タイトル Marinobacter antarcticus sp. nov., a halotolerant bacterium isolated from Antarctic intertidal sandy sediment.
ジャーナル Int J Syst Evol Microbiol
Abstract A Gram-staining-negative, aerobic, motile, oxidase- and catalase-positive, rod-shaped strain, designated ZS2-30(T), was isolated from Antarctic intertidal sandy sediment. The strain grew at 4-35 °C (optimum, 25 °C) and in 0-25% (w/v) NaCl (optimum, 3.0-4.0%). It could reduce nitrate to nitrite and hydrolyse Tween 80. The predominant cellular fatty acids of strain ZS2-30(T) were summed feature 3 (C(16:1)ω7c and/or C(16:1)ω6c), C(16:0), C(18:1)ω9c, C(16:1)ω9c, C(12:0) 3-OH and C(12:0). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unidentified aminophospholipid. The genomic DNA G+C content of strain ZS2-30(T) was 55.8 mol%. Analyses of 16S rRNA gene sequences revealed that strain ZS2-30(T) was affiliated with the genus Marinobacter. It showed highest 16S rRNA gene sequence similarities to the type strains of three species of the genus Marinobacter, namely Marinobacter maritimus (98.3%), Marinobacter psychrophilus (98.1%) and Marinobacter goseongensis (97.1%), but the DNA-DNA relatedness values between strain ZS2-30(T) and the above three species were all lower than 45%. Moreover, strain ZS2-30(T) could be distinguished from closely related species of the genus Marinobacter by various phenotypic properties. Based on this taxonomic study using a polyphasic approach, strain ZS2-30(T) is considered to represent a novel species in the genus Marinobacter, for which the name Marinobacter antarcticus sp. nov. is proposed. The type strain of Marinobacter antarcticus is ZS2-30(T) ( = CGMCC 1.10835(T) = KCTC 23684(T)).
巻・号 62(Pt 8)
ページ 1838-1844
公開日 2012-8-1
DOI 10.1099/ijs.0.035774-0
PMID 21984673
MeSH Antarctic Regions Bacterial Typing Techniques Base Composition DNA, Bacterial / genetics Fatty Acids / analysis Geologic Sediments / microbiology* Marinobacter / classification* Marinobacter / genetics Marinobacter / isolation & purification Molecular Sequence Data Phospholipids / analysis Phylogeny* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA
IF 2.415
引用数 13
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 12521