| RRC ID |
21325
|
| 著者 |
Ogiso T, Ueno C, Dianou D, Huy TV, Katayama A, Kimura M, Asakawa S.
|
| タイトル |
Methylomonas koyamae sp. nov., a type I methane-oxidizing bacterium from floodwater of a rice paddy field.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
A novel methane-oxidizing bacterium, strain Fw12E-Y(T), was isolated from floodwater of a rice paddy field in Japan. Cells of strain Fw12E-Y(T) were Gram-negative, motile rods with a single polar flagellum and type I intracytoplasmic membrane arrangement. The strain grew only on methane or methanol as sole carbon and energy source. It was able to grow at 10-40 °C (optimum 30 °C), at pH 5.5-7.0 (optimum 6.5) and with 0-0.1% (w/w) NaCl (no growth above 0.5% NaCl). 16S rRNA gene sequence analysis showed that strain Fw12E-Y(T) is related most closely to members of the genus Methylomonas, but at low levels of similarity (95.0-95.4%). Phylogenetic analysis of pmoA and mxaF genes indicated that the strain belongs to the genus Methylomonas (97 and 92 % deduced amino acid sequence identities to Methylomonas methanica S1(T), respectively). The DNA G+C content of strain Fw12E-Y(T) was 57.1 mol%. Chemotaxonomic data regarding the major quinone (MQ-8) and major fatty acids (C(16:1) and C(14:0)) also supported its affiliation to the genus Methylomonas. Based on phenotypic, genomic and phylogenetic data, strain Fw12E-Y(T) is considered to represent a novel species of the genus Methylomonas, for which the name Methylomonas koyamae sp. nov. is proposed. The type strain is Fw12E-Y(T) ( = JCM 16701(T) = NBRC 105905(T) = NCIMB 14606(T)).
|
| 巻・号 |
62(Pt 8)
|
| ページ |
1832-1837
|
| 公開日 |
2012-8-1
|
| DOI |
10.1099/ijs.0.035261-0
|
| PMID |
21984674
|
| MeSH |
Bacterial Typing Techniques
Base Composition
DNA, Bacterial / genetics
Fatty Acids / analysis
Japan
Methane / metabolism
Methylomonas / classification*
Methylomonas / genetics
Methylomonas / isolation & purification
Molecular Sequence Data
Oryza / microbiology*
Phylogeny*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Water Microbiology*
|
| IF |
2.415
|
| 引用数 |
27
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 16701 |
