RRC ID 21352
著者 Williams TJ, Lefèvre CT, Zhao W, Beveridge TJ, Bazylinski DA.
タイトル Magnetospira thiophila gen. nov., sp. nov., a marine magnetotactic bacterium that represents a novel lineage within the Rhodospirillaceae (Alphaproteobacteria).
ジャーナル Int J Syst Evol Microbiol
Abstract A marine, magnetotactic bacterium, designated strain MMS-1(T), was isolated from mud and water from a salt marsh in Woods Hole, Massachusetts, USA, after enrichment in defined oxygen-concentration/redox-gradient medium. Strain MMS-1(T) is an obligate microaerophile capable of chemoorganoheterotrophic and chemolithoautotrophic growth. Optimal growth occurred at pH 7.0 and 24-26 °C. Chemolithoautotrophic growth occurred with thiosulfate as the electron donor and autotrophic carbon fixation was via the Calvin-Benson-Bassham cycle. The G+C content of the DNA of strain MMS-1(T) was 47.2 mol%. Cells were Gram-negative and morphologically variable, with shapes that ranged from that of a lima bean to fully helical. Cells were motile by means of a single flagellum at each end of the cell (amphitrichous). Regardless of whether grown in liquid or semi-solid cultures, strain MMS-1(T) displayed only polar magnetotaxis and possessed a single chain of magnetosomes containing elongated octahedral crystals of magnetite, positioned along the long axis of the cell. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain MMS-1(T) belongs to the family Rhodospirillaceae within the Alphaproteobacteria, and is distantly related to species of the genus Magnetospirillum. Strain MMS-1(T) is therefore considered to represent a novel species of a new genus, for which the name Magnetospira thiophila gen. nov., sp. nov. is proposed. The type strain of Magnetospira thiophila is MMS-1(T) ( = ATCC BAA-1438(T) = JCM 17960(T)).
巻・号 62(Pt 10)
ページ 2443-2450
公開日 2012-10-1
DOI 10.1099/ijs.0.037697-0
PMID 22140150
MeSH Bacterial Typing Techniques Base Composition Chemoautotrophic Growth DNA, Bacterial / genetics Fatty Acids / analysis Magnetosomes / microbiology* Massachusetts Molecular Sequence Data Photosynthesis Phylogeny* RNA, Ribosomal, 16S / genetics Rhodospirillaceae / classification* Rhodospirillaceae / genetics Rhodospirillaceae / isolation & purification Seawater / microbiology* Sequence Analysis, DNA Water Microbiology* Wetlands
IF 2.415
引用数 32
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 17960