| RRC ID |
21414
|
| Author |
Hou J, Liu Y, Lu Z, Liu X, Liu J.
|
| Title |
Biochemical characterization of RNase HII from Aeropyrum pernix.
|
| Journal |
Acta Biochim Biophys Sin (Shanghai)
|
| Abstract |
Aeropyrum pernix contains one homolog of ribonuclease H (RNase H), A. pernix RNase HII (Ape-RNase HII). Activity characterization showed that Ape-RNase HII exhibited the highest activity in the presence of 5 mM Mn(2+), 1 mM Co(2+), or 10 mM Mg(2+), respectively; however, its cleavage efficiencies at different cleavage sites for Mn(2+) and Mg(2+) were different. Ape-RNase HII cleaved 12-bp RNA/DNA substrates at multiple sites and the optimum pH value was 11.0. Moreover, 16-bp DNA-r4-DNA/DNA and 13-bp DNA-r1-DNA/DNA chimeric substrates were cleaved at DNA-RNA junction. Ape-RNase HII was thermostable and the stabilization was enhanced with increased salt concentration. This work is believed to be the first in vitro functional study of Ape-RNase HII and the results should contribute to the analysis of RNase H of other archaeal species.
|
| Volume |
44(4)
|
| Pages |
339-46
|
| Published |
2012-4-1
|
| DOI |
10.1093/abbs/gms011
|
| PII |
gms011
|
| PMID |
22366566
|
| MeSH |
Aeropyrum / enzymology*
Aeropyrum / genetics
Amino Acid Sequence
Archaeal Proteins / genetics
Archaeal Proteins / metabolism*
Binding Sites / genetics
Biocatalysis / drug effects
Cobalt / pharmacology
DNA, Archaeal / genetics
DNA, Archaeal / metabolism
Electrophoresis, Polyacrylamide Gel
Enzyme Stability
Hydrogen-Ion Concentration
Kinetics
Manganese / pharmacology
Molecular Sequence Data
RNA, Archaeal / genetics
RNA, Archaeal / metabolism
Recombinant Proteins / metabolism
Ribonuclease H / genetics
Ribonuclease H / metabolism*
Sequence Homology, Amino Acid
Substrate Specificity
Temperature
|
| IF |
2.836
|
| Times Cited |
6
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| General Microbes |
JCM 9820 |
