Reference - Detail
RRC ID | 21418 |
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Author | Kim SM, Paek KH, Lee SB. |
Title | Characterization of NADP+-specific L-rhamnose dehydrogenase from the thermoacidophilic Archaeon Thermoplasma acidophilum. |
Journal | Extremophiles |
Abstract |
Thermoplasma acidophilum utilizes L-rhamnose as a sole carbon source. To determine the metabolic pathway of L-rhamnose in Archaea, we identified and characterized L-rhamnose dehydrogenase (RhaD) in T. acidophilum. Ta0747P gene, which encodes the putative T. acidophilum RhaD (Ta_RhaD) enzyme belonging to the short-chain dehydrogenase/reductase family, was expressed in E. coli as an active enzyme catalyzing the oxidation of L-rhamnose to L-rhamnono-1,4-lactone. Analysis of catalytic properties revealed that Ta_RhaD oxidized L-rhamnose, L-lyxose, and L-mannose using only NADP(+) as a cofactor, which is different from NAD(+)/NADP(+)-specific bacterial RhaDs and NAD(+)-specific eukaryal RhaDs. Ta_RhaD showed the highest activity toward L-rhamnose at 60 °C and pH 7. The K (m) and k (cat) values were 0.46 mM, 1,341.3 min(-1) for L-rhamnose and 0.1 mM, 1,027.2 min(-1) for NADP(+), respectively. Phylogenetic analysis indicated that branched lineages of archaeal RhaD are quite distinct from those of Bacteria and Eukarya. This is the first report on the identification and characterization of NADP(+)-specific RhaD. |
Volume | 16(3) |
Pages | 447-54 |
Published | 2012-5-1 |
DOI | 10.1007/s00792-012-0444-1 |
PMID | 22481639 |
MeSH | Archaeal Proteins / chemistry* Archaeal Proteins / genetics Archaeal Proteins / metabolism Bacterial Proteins / chemistry Bacterial Proteins / genetics Bacterial Proteins / metabolism Carbohydrate Dehydrogenases / chemistry* Carbohydrate Dehydrogenases / genetics Carbohydrate Dehydrogenases / metabolism Escherichia coli / genetics Escherichia coli / metabolism Gene Expression NADP / chemistry* NADP / genetics NADP / metabolism Phylogeny Recombinant Proteins / chemistry Recombinant Proteins / genetics Recombinant Proteins / metabolism Rhamnose / chemistry Rhamnose / genetics Rhamnose / metabolism Thermoplasma / enzymology* Thermoplasma / genetics |
IF | 2.462 |
Times Cited | 13 |
WOS Category | MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
Resource | |
General Microbes | JCM 9062 |