論文 - 詳細
| RRC ID | 21434 |
|---|---|
| 著者 | Hosoki K, Nakamura A, Nagao M, Hiraguchi Y, Tanida H, Tokuda R, Wada H, Nobori T, Suga S, Fujisawa T. |
| タイトル | Staphylococcus aureus directly activates eosinophils via platelet-activating factor receptor. |
| ジャーナル | J Leukoc Biol |
| Abstract |
Colonization by SA is associated with exacerbation of AD. Eosinophilic inflammation is a cardinal pathological feature of AD, but little is known about possible direct interaction between SA and eosinophils. PAFR appears to be involved in phagocytosis of Gram-positive bacteria by leukocytes. The objective of this study was to investigate whether SA directly induces eosinophil effector functions via PAFR in the context of AD pathogenesis. Peripheral blood eosinophils were cultured with heat-killed SA, and EDN release, superoxide generation, and adhesion to fibronectin-coated plates were measured. Cytokines, released in the supernatants, were quantified by multiplex bead immunoassays. FISH-labeled SA was incubated with eosinophils and visualized by confocal laser-scanning microscopy. PAFR-blocking peptide and PAFR antagonists were tested for inhibitory effects on SA-induced reactions. SA induced EDN release and superoxide generation by eosinophils in a dose-dependent manner. IL-5 significantly enhanced SA-induced EDN release. IL-5 and IL-17A significantly enhanced SA-induced superoxide generation. SA enhanced eosinophil adhesion to fibronectin, which was blocked by anti-CD49d, and induced eosinophil secretion of various cytokines/chemokines (IL-2R, IL-9, TNFR, IL-1 β, IL-17A, IP-10, TNF-α, PDGF-bb, VEGF, and FGF-basic). After incubation of eosinophils with SA, FISH-labeled SA was visualized in the eosinophils' cytoplasm, indicating phagocytosis. A PAFR-blocking peptide and two PAFR antagonists completely inhibited those reactions. In conclusion, SA directly induced eosinophil activation via PAFR. Blockade of PAFR may be a novel, therapeutic approach for AD colonized by SA. |
| 巻・号 | 92(2) |
| ページ | 333-41 |
| 公開日 | 2012-8-1 |
| DOI | 10.1189/jlb.0112009 |
| PII | jlb.0112009 |
| PMID | 22595142 |
| MeSH | Adult Cells, Cultured Dermatitis, Atopic / immunology Dermatitis, Atopic / metabolism Dermatitis, Atopic / microbiology Eosinophils / immunology* Eosinophils / metabolism* Eosinophils / microbiology Humans Platelet Membrane Glycoproteins / antagonists & inhibitors Platelet Membrane Glycoproteins / metabolism Platelet Membrane Glycoproteins / physiology* Receptors, G-Protein-Coupled / antagonists & inhibitors Receptors, G-Protein-Coupled / metabolism Receptors, G-Protein-Coupled / physiology* Staphylococcal Skin Infections / immunology* Staphylococcal Skin Infections / metabolism* Staphylococcal Skin Infections / microbiology Staphylococcus aureus / immunology |
| IF | 3.757 |
| 引用数 | 13 |
| WOS 分野 | IMMUNOLOGY HEMATOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 2151 |