Reference - Detail
| RRC ID | 21437 |
|---|---|
| Author | Yokoi T, Isobe K, Yoshimura T, Hemmi H. |
| Title | Archaeal phospholipid biosynthetic pathway reconstructed in Escherichia coli. |
| Journal | Archaea |
| Abstract |
A part of the biosynthetic pathway of archaeal membrane lipids, comprised of 4 archaeal enzymes, was reconstructed in the cells of Escherichia coli. The genes of the enzymes were cloned from a mesophilic methanogen, Methanosarcina acetivorans, and the activity of each enzyme was confirmed using recombinant proteins. In vitro radioassay showed that the 4 enzymes are sufficient to synthesize an intermediate of archaeal membrane lipid biosynthesis, that is, 2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate, from precursors that can be produced endogenously in E. coli. Introduction of the 4 genes into E. coli resulted in the production of archaeal-type lipids. Detailed liquid chromatography/electron spray ionization-mass spectrometry analyses showed that they are metabolites from the expected intermediate, that is, 2,3-di-O-geranylgeranyl-sn-glycerol and 2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphoglycerol. The metabolic processes, that is, dephosphorylation and glycerol modification, are likely catalyzed by endogenous enzymes of E. coli. |
| Volume | 2012 |
| Pages | 438931 |
| Published | 2012-1-1 |
| DOI | 10.1155/2012/438931 |
| PMID | 22645416 |
| PMC | PMC3357500 |
| MeSH | Archaeal Proteins / genetics Archaeal Proteins / metabolism* Biosynthetic Pathways / genetics* Chromatography, Liquid Cloning, Molecular Escherichia coli / chemistry Escherichia coli / genetics* Escherichia coli / metabolism* Membrane Lipids / biosynthesis Metabolic Engineering Methanosarcina / enzymology* Methanosarcina / genetics Models, Biological Phospholipids / biosynthesis* Recombinant Proteins / genetics Recombinant Proteins / metabolism Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| IF | 3.379 |
| Times Cited | 15 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Wikipedia |
| Total number of mentions | 5 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| General Microbes | JCM 12185 |