| RRC ID |
21452
|
| Author |
Duangmal K, Mingma R, Pathom-aree W, Niyomvong N, Inahashi Y, Matsumoto A, Thamchaipenet A, Takahashi Y.
|
| Title |
Microbispora thailandensis sp. nov., an actinomycete isolated from cave soil.
|
| Journal |
J Antibiot (Tokyo)
|
| Abstract |
The taxonomic position of actinomycete strain NN276(T), isolated from cave soil, was studied using the polyphasic taxonomic approach. A phylogenetic tree based on 16S ribosomal RNA (rRNA) gene sequences showed that the isolate formed a distinct evolutionary linage with the genus Microbispora, with M. mesophila JCM 3151(T) as its closest phylogenetic neighbor (97.9% similarity). The organism contained meso-diaminopimelic acid and the N-acetyl type of peptidoglycan. Madurose was detected in the whole-cell hydrolasate. The predominant menaquinones were MK-9(H(4)), MK-9(H(2)) and MK-9. Mycolic acids were not detected. Major phospholipids were diphosphatidylglycerol, hydroxy-phosphatidylethanolamine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The major cellular fatty acid was iso-C(16: 0) and G+C content 70 mol%. DNA-DNA hybridization demonstrated that the isolate was distinct from M. mesophila JCM 3151(T). On the basis of phenotypic and genotypic data, it is proposed that strain NN276(T) represents a novel species of the genus Microbispora, hence the name Microbispora thailandensis sp. nov. The type strain is strain NN276(T) (=BCC 41490(T)=NRRL B-24806(T)=NBRC 107569(T)).
|
| Volume |
65(10)
|
| Pages |
491-4
|
| Published |
2012-10-1
|
| DOI |
10.1038/ja.2012.57
|
| PII |
ja201257
|
| PMID |
22781279
|
| MeSH |
Actinomycetales / chemistry
Actinomycetales / classification*
Actinomycetales / genetics
Actinomycetales / isolation & purification*
Bacterial Typing Techniques
Base Composition
Caves / microbiology*
Cluster Analysis
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Diaminopimelic Acid / analysis
Fatty Acids / analysis
Microscopy, Electron
Molecular Sequence Data
Nucleic Acid Hybridization
Peptidoglycan / chemistry
Phospholipids / analysis
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Soil Microbiology*
Vitamin K 2 / analysis
|
| IF |
2.668
|
| Times Cited |
10
|
|
WOS Category
|
PHARMACOLOGY & PHARMACY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
IMMUNOLOGY
|
| Resource |
| General Microbes |
JCM 3151 |
