| RRC ID |
21454
|
| Author |
Furuhata K, Edagawa A, Miyamoto H, Morimoto Y, Fukuyama M.
|
| Title |
Identification of Legionella rubrilucens isolated from a hot spring for foot-soaking in Niigata, Japan.
|
| Journal |
Biocontrol Sci
|
| Abstract |
In May 2011, strain HYNE-20 (=JCM 17837) was isolated from a sample of hot spring water from a foot spa in Niigata, Japan, by a plating method using glycine vancomycin polymyxin B cycloheximide α-ketoglutarate (GVPCα) medium at 36°C for 7 d. The 16S rDNA sequences (1,469bp) of this strain (accession number: AB638719) had high (99.7%) similarity to Legionella rubrilucens, and we identified that this strain was indeed Legionella rubrilucens. When this strain was cultured on buffered charcoal yeast extract α-ketoglutarate (BCYEα) agar at 36°C for 7 d, it exhibited red autofluorescence under UV light (365 nm) . The dominant cellular fatty acids of the strain HYNE-20 were 16:1ω7c (29.9%) , and the guanine-plus-cytosine (G+C) content of DNA was 49.0 mol%. This is the first report that Legionella rubrilucens was isolated from a hot spring for foot soaking.
|
| Volume |
17(2)
|
| Pages |
101-5
|
| Published |
2012-6-1
|
| DOI |
10.4265/bio.17.101
|
| PII |
DN/JST.JSTAGE/bio/17.101
|
| PMID |
22790847
|
| MeSH |
Base Sequence
Culture Media
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / genetics
Fluorescence
Health Resorts
Hot Springs / microbiology*
Japan
Legionella / classification*
Legionella / genetics
Legionella / isolation & purification
Legionellosis / microbiology
Molecular Sequence Data
Phenotype
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Water Microbiology*
|
| IF |
0.833
|
| Times Cited |
2
|
|
WOS Category
|
BIOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| Resource |
| General Microbes |
JCM 7565
JCM 17837 |
