RRC ID 21460
著者 Iino T, Suzuki R, Kosako Y, Ohkuma M, Komagata K, Uchimura T.
タイトル Acetobacter okinawensis sp. nov., Acetobacter papayae sp. nov., and Acetobacter persicus sp. nov.; novel acetic acid bacteria isolated from stems of sugarcane, fruits, and a flower in Japan.
ジャーナル J Gen Appl Microbiol
Abstract Eleven strains of acetic acid bacteria were isolated from stems of sugarcane, fruits, and a flower in Japan. The isolates were separated into three groups, Groups I, II, and III, in the genus Acetobacter according to phylogenetic analysis based on 16S rRNA sequences. The isolates had sequence similarities of 99.8-100% within the Group, 99.3-99.6% to those of the type strains of each related Acetobacter species, and less than 98.4% to those of the type strains of other Acetobacter species. Genomic DNA G+C contents of Groups I, II, and III were 59.2-59.4, 60.5-60.7, and 58.7-58.9 mol%, respectively. The isolates in the Group showed high values of DNA-DNA relatedness to each other, but low values less than 46% to the type strains of related Acetobacter species. A good correlation was found between the three Groups and groups based on DNA G+C contents and DNA-DNA relatedness. All the strains had Q-9 as the main component, and Q-8 and Q-10 as minor components. The isolates in the three Groups did not completely match with any Acetobacter species on catalase reaction, the production of ketogluconic acids from D-glucose, growth on ammoniac nitrogen with ethanol (Hoyer-Frateur medium and Frateur modified Hoyer medium), growth on 30% (w/v) D-glucose, growth in 10% (v/v) ethanol, or DNA G+C contents. On the basis of phylogenetic relationships in the genus Acetobacter and chemosystematic and phenotypic characteristics, the three Groups were regarded as novel species in the genus Acetobacter. Acetobacter okinawensis sp. nov. is proposed for Group I, Acetobacter papayae sp. nov. for Group II, and Acetobacter persicus sp. nov. for Group III.
巻・号 58(3)
ページ 235-43
公開日 2012-1-1
DOI 10.2323/jgam.58.235
PII DN/JST.JSTAGE/jgam/58.235
PMID 22878741
MeSH Acetic Acid / metabolism* Acetobacter / classification* Acetobacter / genetics Acetobacter / isolation & purification* Acetobacter / metabolism Bacterial Typing Techniques Base Composition Cluster Analysis DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Ethanol / metabolism Flowers / microbiology Fruit / microbiology Glucose / metabolism Japan Molecular Sequence Data Nucleic Acid Hybridization Phylogeny Plant Stems / microbiology Quinones / analysis RNA, Ribosomal, 16S / genetics Saccharum / microbiology* Sequence Analysis, DNA
IF 1.442
引用数 17
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
リソース情報
一般微生物 JCM 7639 JCM 7641 JCM 11197 JCM 15829 JCM 17121 JCM 17273 JCM 17274 JCM 25077 JCM 25143 JCM 25144 JCM 25146 JCM 25147 JCM 25330 JCM 25332 JCM 25387 JCM 25390 JCM 25401 JCM 25402