| RRC ID |
21469
|
| 著者 |
Neal-McKinney JM, Lu X, Duong T, Larson CL, Call DR, Shah DH, Konkel ME.
|
| タイトル |
Production of organic acids by probiotic lactobacilli can be used to reduce pathogen load in poultry.
|
| ジャーナル |
PLoS One
|
| Abstract |
Probiotic Lactobacillus can be used to reduce the colonization of pathogenic bacteria in food animals, and therefore reduce the risk of foodborne illness to consumers. As a model system, we examined the mechanism of protection conferred by Lactobacillus species to inhibit C. jejuni growth in vitro and reduce colonization in broiler chickens. Possible mechanisms for the reduction of pathogens by lactobacilli include: 1) stimulation of adaptive immunity; 2) alteration of the cecal microbiome; and, 3) production of inhibitory metabolites, such as organic acids. The Lactobacillus species produced lactic acid at concentrations sufficient to kill C. jejuni in vitro. We determined that lactic acid produced by Lactobacillus disrupted the membrane of C. jejuni, as judged by biophotonics. The spectral features obtained using Fourier-transform infrared (FT-IR) and Raman spectroscopy techniques were used to accurately predict bacterial viability and differentiate C. jejuni samples according to lactic acid treatment. FT-IR spectral features of C. jejuni and Lactobacillus grown in co-culture revealed that the metabolism was dominated by Lactobacillus prior to the killing of C. jejuni. Based on our results, the development of future competitive exclusion strategies should include the evaluation of organic acid production.
|
| 巻・号 |
7(9)
|
| ページ |
e43928
|
| 公開日 |
2012-1-1
|
| DOI |
10.1371/journal.pone.0043928
|
| PII |
PONE-D-12-15462
|
| PMID |
22962594
|
| PMC |
PMC3433458
|
| MeSH |
Administration, Oral
Animals
Bacterial Load / drug effects
Campylobacter Infections / microbiology
Campylobacter Infections / prevention & control*
Campylobacter Infections / veterinary*
Campylobacter jejuni / drug effects
Campylobacter jejuni / growth & development
Chickens / microbiology*
Coculture Techniques
Lactic Acid / biosynthesis
Lactic Acid / pharmacology
Lactobacillus / metabolism*
Microbial Viability / drug effects
Poultry Diseases / microbiology
Poultry Diseases / prevention & control*
Probiotics / administration & dosage*
Probiotics / therapeutic use
Spectroscopy, Fourier Transform Infrared
Spectrum Analysis, Raman
|
| IF |
2.74
|
| 引用数 |
102
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 5810 |
