| RRC ID |
21484
|
| 著者 |
Mou YZ, Qiu XX, Zhao ML, Cui HL, Oh D, Dyall-Smith ML.
|
| タイトル |
Halohasta litorea gen. nov. sp. nov., and Halohasta litchfieldiae sp. nov., isolated from the Daliang aquaculture farm, China and from Deep Lake, Antarctica, respectively.
|
| ジャーナル |
Extremophiles
|
| Abstract |
Two halophilic archaeal strains, R30(T) and tADL(T), were isolated from an aquaculture farm in Dailing, China, and from Deep Lake, Antarctica, respectively. Both have rod-shaped cells that lyse in distilled water, stain Gram-negative and form red-pigmented colonies. They are neutrophilic, require >120 g/l NaCl and 48-67 g/l MgCl(2) for growth but differ in their optimum growth temperatures (30 °C, tADL(T) vs. 40 °C, R30(T)). The major polar lipids were typical for members of the Archaea but also included a major glycolipid chromatographically identical to sulfated mannosyl glucosyl diether (S-DGD-1). The 16S rRNA gene sequences of the two strains are 97.4 % identical, show most similarity to genes of the family Halobacteriaceae, and cluster together as a distinct clade in phylogenetic tree reconstructions. The rpoB' gene similarity between strains R30(T) and tADL(T) is 92.9 % and less to other halobacteria. Their DNA G + C contents are 62.4-62.9 mol % but DNA-DNA hybridization gives a relatedness of only 44 %. Based on phenotypic, chemotaxonomic and phylogenetic properties, we describe two new species of a novel genus, represented by strain R30(T) (= CGMCC 1.10593(T) = JCM 17270(T)) and strain tADL(T) (= JCM 15066(T) = DSMZ 22187(T)) for which we propose the names Halohasta litorea gen. nov., sp. nov. and Halohasta litchfieldiae sp. nov., respectively.
|
| 巻・号 |
16(6)
|
| ページ |
895-901
|
| 公開日 |
2012-11-1
|
| DOI |
10.1007/s00792-012-0485-5
|
| PMID |
23052830
|
| MeSH |
Antarctic Regions
Archaeal Proteins / genetics
China
DNA, Archaeal / chemistry
Halobacteriaceae / classification*
Halobacteriaceae / cytology
Halobacteriaceae / isolation & purification*
Phylogeny
RNA, Ribosomal, 16S / genetics
|
| IF |
2.462
|
| 引用数 |
26
|
|
WOS 分野
|
MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 9738
JCM 15066
JCM 17270 |
