RRC ID |
21488
|
著者 |
Jojima T, Igari T, Gunji W, Suda M, Inui M, Yukawa H.
|
タイトル |
Identification of a HAD superfamily phosphatase, HdpA, involved in 1,3-dihydroxyacetone production during sugar catabolism in Corynebacterium glutamicum.
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ジャーナル |
FEBS Lett
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Abstract |
Corynebacterium glutamicum produces 1,3-dihydroxyacetone (DHA) as metabolite of sugar catabolism but the responsible enzyme is yet to be identified. Using a transposon mutant library, the gene hdpA (cgR_2128) was shown to encode a haloacid dehalogenase superfamily member that catalyzes dephosphorylation of dihydroxyacetone phosphate to produce DHA. Inactivation of hdpA led to a drastic decrease in DHA production from each of glucose, fructose, and sucrose, indicating that HdpA is the main enzyme responsible for DHA production from sugars in C. glutamicum. Confirmation of DHA production via dihydroxyacetone phosphatase finally confirms a long-speculated route through which bacteria produce DHA.
|
巻・号 |
586(23)
|
ページ |
4228-32
|
公開日 |
2012-11-30
|
DOI |
10.1016/j.febslet.2012.10.028
|
PII |
S0014-5793(12)00811-3
|
PMID |
23108048
|
MeSH |
Bacterial Proteins / genetics
Bacterial Proteins / metabolism
Corynebacterium glutamicum / enzymology*
Dihydroxyacetone / biosynthesis*
Dihydroxyacetone / metabolism
Phosphoric Monoester Hydrolases / genetics
Phosphoric Monoester Hydrolases / metabolism*
|
IF |
3.057
|
引用数 |
16
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
リソース情報 |
一般微生物 |
JCM 18229 |