RRC ID 21514
Author Yamaguchi K, Feril LB Jr, Tachibana K, Takahashi A, Matsuo M, Endo H, Harada Y, Nakayama J.
Title Ultrasound-mediated interferon β gene transfection inhibits growth of malignant melanoma.
Journal Biochem. Biophys. Res. Commun.
Abstract We investigated the effects of ultrasound-mediated transfection (sonotransfection) of interferon β (IFN-β) gene on melanoma (C32) both in vitro and in vivo. C32 cells were sonotransfected with IFN-β in vitro. Subcutaneous C32 tumors in mice were sonicated weekly immediately after intra-tumor injection with IFN-β genes mixed with microbubbles. Successful sonotransfection with IFN-β gene in vitro was confirmed by ELISA, which resulted in C32 growth inhibition. In vivo, the growth ratio of tumors transfected with IFN-β gene was significantly lower than the other experimental groups. These results may lead to a new method of treatment against melanoma and other hard-to-treat cancers.
Volume 411(1)
Pages 137-42
Published 2011-7-22
DOI 10.1016/j.bbrc.2011.06.115
PII S0006-291X(11)01095-3
PMID 21723255
MeSH Animals Cell Line, Tumor Female Genetic Therapy / methods* Humans Interferon-beta / genetics* Interferon-beta / metabolism Melanoma / therapy* Mice Mice, Inbred BALB C Mice, Nude Skin Neoplasms / genetics* Transfection / methods* Ultrasonics
IF 2.705
Times Cited 10
DNA material pCMV (Hu beta) (RDB01681)