RRC ID 21520
Author Chen Q, Osada K, Ishii T, Oba M, Uchida S, Tockary TA, Endo T, Ge Z, Kinoh H, Kano MR, Itaka K, Kataoka K.
Title Homo-catiomer integration into PEGylated polyplex micelle from block-catiomer for systemic anti-angiogenic gene therapy for fibrotic pancreatic tumors.
Journal Biomaterials
Abstract Homo-poly{N'-[N-(2-aminoethyl)-2-aminoehtyl]aspartamide} [PAsp(DET), H] was attempted to integrate into poly (ethylene glycol) (PEG)-b-PAsp(DET)] (B) formulated polyplex micelle with the aim of enhancing cell transfection efficiency for PEGylated polyplex micelle via H integration. In vitro evaluations verified H integration of potent stimulation in enhancing cell-transfecting activity of PEGylated polyplex micelles via promoted cellular uptake and facilitated endosome escape. In vivo anti-angiogenic tumor suppression evaluations validated the feasibility of H integration in promoting gene transfection to the affected cells via systemic administration, where loaded anti-angiogenic gene remarkably expressed in the tumor site, thereby imparting significant inhibitory effect on the growth of vascular endothelial cells, ultimately leading to potent tumor growth suppression. These results demonstrated potency of H integration for enhanced transfection activity and potential usage in systemic applications, which could have important implications on the strategic use of H integration in the non-viral gene carrier design.
Volume 33(18)
Pages 4722-30
Published 2012-6-1
DOI 10.1016/j.biomaterials.2012.03.017
PII S0142-9612(12)00289-X
PMID 22444644
MeSH Animals Cell Line Cell Survival / drug effects Flow Cytometry Genetic Therapy / methods* Humans Mice Mice, Nude Micelles* Microscopy, Electron, Transmission Nanoparticles / adverse effects Nanoparticles / chemistry* Pancreatic Neoplasms / therapy* Polyethylene Glycols / chemistry*
IF 10.317
Times Cited 40
DNA material pCAcc-Luc+ (RDB02443)