RRC ID 21524
Author Girdwood D, Robertson M, Gordon C.
Title Constitutively active Cullin-RING-Ligases fail to rescue loss of NEDD8 conjugation in Schizosaccharomyces pombe.
Journal FEBS Lett.
Abstract In fission yeast, the only known essential function of Ned8p is the modification of the cullin, Pcu1p, and subsequent Cullin-RING-Ligase (CRL) activation and substrate ubiquitination. We show here that a functional Pcu1p mutant, deleted for its C-terminal autoinhibitory domain, which negates the requirement of neddylation for ligase activity, is unable to rescue the loss of neddylation. These findings suggest that the neddylation of non-cullin substrate(s) are required for Schizosaccharomyces pombe viability.
Volume 586(10)
Pages 1522-8
Published 2012-5-21
DOI 10.1016/j.febslet.2012.04.011
PII S0014-5793(12)00289-X
PMID 22673520
MeSH Base Sequence Cullin Proteins / physiology* DNA Primers Ligases / metabolism* Molecular Sequence Data Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / metabolism*
IF 2.999
Times Cited 2
WOS Category BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
DNA material pDUAL-FFH1c (RDB06142) S. pombe FLAG2-His SpFFH20F06 (SPW048126) SpFFH47B10 (SPW058834)