RRC ID 21536
Author Araki Y, Hamafuji T, Noguchi C, Shimizu N.
Title Efficient recombinant production in mammalian cells using a novel IR/MAR gene amplification method.
Journal PLoS ONE
Abstract We previously found that plasmids bearing a mammalian replication initiation region (IR) and a nuclear matrix attachment region (MAR) efficiently initiate gene amplification and spontaneously increase their copy numbers in animal cells. In this study, this novel method was applied to the establishment of cells with high recombinant antibody production. The level of recombinant antibody expression was tightly correlated with the efficiency of plasmid amplification and the cytogenetic appearance of the amplified genes, and was strongly dependent on cell type. By using a widely used cell line for industrial protein production, CHO DG44, clones expressing very high levels of antibody were easily obtained. High-producer clones stably expressed the antibody over several months without eliciting changes in both the protein expression level and the cytogenetic appearance of the amplified genes. The integrity and reactivity of the protein produced by this method was fine. In serum-free suspension culture, the specific protein production rate in high-density cultures was 29.4 pg/cell/day. In conclusion, the IR/MAR gene amplification method is a novel and efficient platform for recombinant antibody production in mammalian cells, which rapidly and easily enables the establishment of stable high-producer cell clone.
Volume 7(7)
Pages e41787
Published 2012
DOI 10.1371/journal.pone.0041787
PII PONE-D-12-11763
PMID 22844523
PMC PMC3402416
MeSH Animals CHO Cells Cell Line, Tumor Cricetinae Cricetulus DNA Replication / genetics* DNA, Recombinant / biosynthesis* DNA, Recombinant / genetics* Genetic Engineering / methods* Humans Immunoglobulin G / biosynthesis Immunoglobulin G / genetics Matrix Attachment Regions / genetics* Nucleic Acid Amplification Techniques / methods* Plasmids / genetics* Transfection
IF 2.766
Times Cited 13
WOS Category BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Resource
DNA material pAxEFwtit2 (RDB05215) pxCAG (RDB02546)