Reference - Detail
| RRC ID | 21538 |
|---|---|
| Author | Kobayashi K, Saito K, Ishitani R, Ito K, Nureki O. |
| Title | Structural basis for translation termination by archaeal RF1 and GTP-bound EF1α complex. |
| Journal | Nucleic Acids Res |
| Abstract |
When a stop codon appears at the ribosomal A site, the class I and II release factors (RFs) terminate translation. In eukaryotes and archaea, the class I and II RFs form a heterodimeric complex, and complete the overall translation termination process in a GTP-dependent manner. However, the structural mechanism of the translation termination by the class I and II RF complex remains unresolved. In archaea, archaeal elongation factor 1 alpha (aEF1α), a carrier GTPase for tRNA, acts as a class II RF by forming a heterodimeric complex with archaeal RF1 (aRF1). We report the crystal structure of the aRF1·aEF1α complex, the first active class I and II RF complex. This structure remarkably resembles the tRNA·EF-Tu complex, suggesting that aRF1 is efficiently delivered to the ribosomal A site, by mimicking tRNA. It provides insights into the mechanism that couples GTP hydrolysis by the class II RF to stop codon recognition and peptidyl-tRNA hydrolysis by the class I RF. We discuss the different mechanisms by which aEF1α recognizes aRF1 and aPelota, another aRF1-related protein and molecular evolution of the three functions of aEF1α. |
| Volume | 40(18) |
| Pages | 9319-28 |
| Published | 2012-10-1 |
| DOI | 10.1093/nar/gks660 |
| PII | gks660 |
| PMID | 22772989 |
| PMC | PMC3467058 |
| MeSH | Archaeal Proteins / chemistry* Archaeal Proteins / metabolism Codon, Terminator Guanosine Triphosphate / chemistry* Guanosine Triphosphate / metabolism Models, Molecular Peptide Chain Termination, Translational* Peptide Elongation Factor 1 / chemistry* Peptide Elongation Factor 1 / metabolism Peptide Elongation Factor Tu / chemistry Peptide Termination Factors / chemistry* Peptide Termination Factors / metabolism RNA, Transfer / chemistry Structural Homology, Protein |
| IF | 11.502 |
| Times Cited | 22 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Wikipedia |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | Genomic DNA of Aeropyrum pernix JCM 9820T (JGD07499) |
| General Microbes | |