RRC ID 2156
著者 Iwase M, Luo J, Bi E, Toh-e A.
タイトル Shs1 plays separable roles in septin organization and cytokinesis in Saccharomyces cerevisiae.
ジャーナル Genetics
Abstract In Saccharomyces cerevisiae, five septins (Cdc3, Cdc10, Cdc11, Cdc12, and Shs1/Sep7) form the septin ring at the bud neck during vegetative growth. We show here that disruption of SHS1 caused cold-sensitive growth in the W303 background, with cells arrested in chains, indicative of a cytokinesis defect. Surprisingly, the other four septins appeared to form an apparently normal septin ring in shs1Delta cells grown under the restrictive condition. We found that Myo1 and Iqg1, two components of the actomyosin contractile ring, and Cyk3, a component of the septum formation, were either delocalized or mislocalized in shs1Delta cells, suggesting that Shs1 plays supportive roles in cytokinesis. We also found that deletion of SHS1 enhanced or suppressed the septin defect in cdc10Delta and cdc11Delta cells, respectively, suggesting that Shs1 is involved in septin organization, exerting different effects on septin-ring assembly, depending on the composition of the septin subunits. Furthermore, we constructed an shs1-100c allele that lacks the coding sequence for the C-terminal 32 amino acids. This allele still displayed the genetic interactions with the septin mutants, but did not show cytokinesis defects as described above, suggesting that the roles of Shs1 in septin organization and cytokinesis are separable.
巻・号 177(1)
ページ 215-29
公開日 2007-9-1
DOI 10.1534/genetics.107.073007
PII genetics.107.073007
PMID 17603111
PMC PMC2013704
MeSH Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Cytokinesis* Cytoskeletal Proteins / genetics Cytoskeletal Proteins / metabolism* GTP Phosphohydrolases / genetics GTP Phosphohydrolases / metabolism* Membrane Proteins / genetics Membrane Proteins / metabolism* Saccharomyces cerevisiae / cytology* Saccharomyces cerevisiae / physiology Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism* Two-Hybrid System Techniques
IF 4.015
引用数 40
WOS 分野 GENETICS & HEREDITY
リソース情報
酵母 BY22476 BY22486 BY22487 BY22479 BY22480 BY22484