| RRC ID |
21637
|
| 著者 |
Chonudomkul D, Yongmanitchai W, Theeragool G, Kawachi M, Kasai F, Kaya K, Watanabe MM.
|
| タイトル |
Morphology, genetic diversity, temperature tolerance and toxicity of Cylindrospermopsis raciborskii (Nostocales, Cyanobacteria) strains from Thailand and Japan.
|
| ジャーナル |
FEMS Microbiol Ecol
|
| Abstract |
Cylindrospermopsis raciborskii is a planktonic, nostocalean cyanobacterium, which produces an alkaloid heptatoxin, cylindrospermopsin. We performed morphological observations, 16S rDNA sequence analysis, PCR fingerprint analysis of short tandemly repeated repetitive (STRR) sequences, temperature tolerances and toxin analysis to characterize 24 strains of this toxic cyanobacterium isolated from Thailand and Japan. All strains shared common morphological traits characteristic of C. raciborskii and showed high 16S rDNA sequence similarity, forming a defined cluster together with the reference strains from Australia. In particular, some of the Thai strains shared 99.9% to 100% similarity with the Australian strains. Various combinations of STRR primers revealed different and unique DNA band patterns among strains of C. raciborskii. The phylogenetic tree revealed two main clusters of C. raciborskii strains, the Thai/Japan-Shinobazugaike cluster (cluster I) and the Japan-Gonoike cluster (cluster II). Cluster I was further divided into two subclusters, A (only Thai strains) and B (one Thai strain and the Japan-Shinobazugaike strains). Thus, the results from 16S rDNA and STRR analyses showed no clear geographical distinction between Japanese and Thai strains and between Thai and Australian strains. Thai strains were separated into adaptive and non-adaptive groups to low temperature (15 and 17.5 degrees C) and Japanese strains were composed of only low-temperature-adaptive ones. The toxin cylindrospermopsin was detected in some strains of cluster I-A and in one strain of cluster II. We conclude that C. raciborskii is a species that has recently begun to invade, and a species with different physiological strains or ecotypes in temperature tolerance; the toxin is synthesized without any relation to phylogenetic or genetic clusters and to geography.
|
| 巻・号 |
48(3)
|
| ページ |
345-55
|
| 公開日 |
2004-6-1
|
| DOI |
10.1016/j.femsec.2004.02.014
|
| PII |
FEM345
|
| PMID |
19712304
|
| MeSH |
Alkaloids
Bacterial Toxins
Cluster Analysis
Cyanobacteria Toxins
Cylindrospermopsis / classification*
Cylindrospermopsis / cytology*
Cylindrospermopsis / isolation & purification
Cylindrospermopsis / radiation effects
DNA Fingerprinting
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Genetic Variation*
Genotype
Geography
Japan
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction / methods
RNA, Ribosomal, 16S / genetics
Repetitive Sequences, Nucleic Acid
Sequence Analysis, DNA
Temperature*
Thailand
Uracil / analogs & derivatives*
Uracil / biosynthesis
|
| IF |
3.675
|
| 引用数 |
108
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 藻類 |
NIES-991
NIES-992
NIES-993
NIES-1040
NIES-1041 |
