RRC ID 21648
Author Spoof L, Neffling MR, Meriluoto J.
Title Fast separation of microcystins and nodularins on narrow-bore reversed-phase columns coupled to a conventional HPLC system.
Journal Toxicon
Abstract Six modern narrow-bore (50 mm length x 2-2.1 mm internal diameter) reversed-phase HPLC columns were tested in the rapid separation of ten microcystins and three nodularins, cyanobacterial peptide hepatotoxins. The columns had either a sub-3 microm particle size or were of monolithic silica technology for high efficiency and rapid run times. A standard low-pressure gradient HPLC equipment with diode-array UV detector was used for the chromatography. The gradient mobile phase consisted of water and acetonitrile, both acidified with trifluoroacetic acid, and the gradient rise times were 1-4 min. Chromatographic performance was assessed by visual judgement and by calculating parameters such as capacity factors, resolution, peak width, selectivity and peak form. Several chromatographic conditions produced excellent results. We recommend sub-3 microm particle-based or monolithic reversed-phase columns for routine use in microcystin and nodularin HPLC analyses.
Volume 55(5)
Pages 954-64
Published 2010-5-1
DOI 10.1016/j.toxicon.2009.06.015
PII S0041-0101(09)00307-9
PMID 19540867
MeSH Bacterial Toxins / isolation & purification* Bacterial Toxins / metabolism Chromatography, High Pressure Liquid / methods Cyanobacteria / metabolism* Marine Toxins / isolation & purification* Marine Toxins / metabolism Microcystins / isolation & purification* Microcystins / metabolism Peptides, Cyclic / isolation & purification* Peptides, Cyclic / metabolism
IF 2.276
Times Cited 31
Algae NIES-107