RRC ID 21698
著者 Yoshida M, Yoshida T, Satomi M, Takashima Y, Hosoda N, Hiroishi S.
タイトル Intra-specific phenotypic and genotypic variation in toxic cyanobacterial Microcystis strains.
ジャーナル J Appl Microbiol
Abstract AIMS:We determined if the intra-specific genetic diversity of Microcystis aeruginosa correlates with phenotypic characteristics.
METHODS AND RESULTS:Microcystis aeruginosa isolates from various Japanese waters were characterized using genetic analyses based on the 16S-23S rDNA internal transcribed spacer (ITS) region and DNA-independent RNA polymerase (rpoC1) gene sequences. In addition, morphological and biochemical properties, and the toxicity of M. aeruginosa strains were determined. We found a correlation in phylogenetic clusters of the ITS region and rpoC1 gene sequences. Using a polyphasic approach, genotypic and phenotypic variations in M. aeruginosa showed that the three genetic lineage groups are comprised of a particular phenotype or subgroup of closely related phenotypes. However, some strains had high phenotypic and genotypic diversity compared to the three lineage groups and did not show distinct lineages; therefore, these strains were designated as the 'complex group'.
CONCLUSIONS:The 'complex group' consisted of genetically and phenotypically incoherent and high diverse populations in M. aeruginosa, although some genotypes or lineages displayed consistent phenotypes.
SIGNIFICANCE AND IMPACT OF THE STUDY:The polyphasic approach combining phenotypic and genetic characterization was effective for comprehending distinct lineages and discriminating the potential complexity of M. aeruginosa populations at the intra-species level.
巻・号 105(2)
ページ 407-15
公開日 2008-8-1
DOI 10.1111/j.1365-2672.2008.03754.x
PII JAM3754
PMID 18355235
MeSH DNA, Ribosomal Spacer / analysis Eutrophication Fresh Water Genetic Variation Genotype Microcystis / classification* Microcystis / genetics Microcystis / metabolism Phenotype RNA, Ribosomal, 16S / analysis Ribotyping Water Microbiology*
IF 3.066
引用数 39
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
リソース情報
藻類 NIES-101 NIES-1070 NIES-1105 NIES-102 NIES-1058 NIES-1091 NIES-112 NIES-604 NIES-1055 NIES-1062 NIES-103 NIES-298