| RRC ID |
216
|
| Author |
Aizawa K, Shimada A, Naruse K, Mitani H, Shima A.
|
| Title |
The medaka midblastula transition as revealed by the expression of the paternal genome.
|
| Journal |
Gene Expr Patterns
|
| Abstract |
At midblastula transition (MBT), zygotic gene transcription is activated, cells become motile and cell division becomes asynchronous. The onset of the medaka (Oryzias latipes) MBT was examined using expressed sequence tag (EST) markers. Among 187 randomly chosen medaka EST markers, 33 EST markers and two genes (eIF-4C and hsc70) showed polymorphisms in terms of insertion/deletions or restriction sites between the two parental inbred strains, one from the northern Japanese population and the other from the southern Japanese population. There was no evidence of zygotic expression of these EST markers before Stage 10 (early blastula stage), whereas expression of 12 genes was found from Stage 11 on. These results suggest that the medaka MBT in terms of first time transcription of paternal genes in the life of the embryo begins at Stage 11 (late blastula stage).
|
| Volume |
3(1)
|
| Pages |
43-7
|
| Published |
2003-3-1
|
| DOI |
10.1016/s1567-133x(02)00075-3
|
| PII |
S1567133X02000753
|
| PMID |
12609601
|
| MeSH |
Animals
Blastula / metabolism*
Gene Expression Profiling*
Oryzias / embryology*
|
| IF |
0.897
|
| Times Cited |
38
|
|
WOS Category
|
GENETICS & HEREDITY
DEVELOPMENTAL BIOLOGY
|
| Resource |
| Medaka |
HNI-II (IB176)
T5 (MT827)
cDNA |
