| RRC ID |
21774
|
| Author |
Kubo T, Kato N, Hosoya K, Kaya K.
|
| Title |
Effective determination method for a cyanobacterial neurotoxin, beta-N-methylamino-L-alanine.
|
| Journal |
Toxicon
|
| Abstract |
We developed a simple and effective analysis procedure that includes pretreatment and determination methods for beta-N-methylamino-l-alanine (BMAA), a cyanobacterial neurotoxin. BMAA may be produced by all known groups of cyanobacteria living in freshwater as well as marine environments. In this paper, we report a novel determination method for BMAA. A cation-exchange resin was effective for the selective concentration of BMAA from cyanobacterial extracts and yielded a high recovery rate. Moreover, liquid chromatography (LC)-electrospray ionization mass spectrometry with a hydrophilic LC column was effective for determining BMAA levels. The quantitation limit for BMAA based on selected ion monitoring (SIM) was determined as 0.5 ng at a signal/noise ratio of 5.
|
| Volume |
51(7)
|
| Pages |
1264-8
|
| Published |
2008-6-1
|
| DOI |
10.1016/j.toxicon.2008.02.015
|
| PII |
S0041-0101(08)00073-1
|
| PMID |
18407310
|
| MeSH |
Amino Acids, Diamino / analysis*
Amino Acids, Diamino / chemistry
Cation Exchange Resins
Chromatography, High Pressure Liquid
Cyanobacteria*
Cyanobacteria Toxins
Excitatory Amino Acid Agonists / analysis*
Excitatory Amino Acid Agonists / chemistry
Neurotoxins / analysis*
Neurotoxins / chemistry
Spectrometry, Mass, Electrospray Ionization
Water Pollutants, Chemical / analysis
Water Pollutants, Chemical / chemistry
|
| IF |
2.201
|
| Times Cited |
48
|
|
WOS Category
|
PHARMACOLOGY & PHARMACY
TOXICOLOGY
|
| Resource |
| Algae |
NIES-88 |
