RRC ID 21784
Author Ohtsuka H, Ogawa S, Kawamura H, Sakai E, Ichinose K, Murakami H, Aiba H.
Title Screening for long-lived genes identifies Oga1, a guanine-quadruplex associated protein that affects the chronological lifespan of the fission yeast Schizosaccharomyces pombe.
Journal Mol Genet Genomics
Abstract Schizosaccharomyces pombe and Saccharomyces cerevisiae are excellent model organisms to study lifespan. We conducted screening to identify novel genes that, when overexpressed, extended the chronological lifespan of fission yeast. We identified seven genes, among which we focused on SPBC16A3.08c. The gene product showed similarity to Ylr150w of S. cerevisiae, which has affinity for guanine-quadruplex nucleic acids (G4). The SPBC16A3.08c product associated with G4 in vitro and complemented the phenotype of an S. cerevisiae Ylr150w deletion mutant. From these results, we proposed that SPBC16A3.08c encoded for a functional homolog of Ylr150w, which we designated ortholog of G4-associated protein (oga1 (+)). oga1 (+) overexpression extended the chronological lifespan and also decreased mating efficiency and caused both high and low temperature-sensitive growth. Deleting oga1 (+) resulted in caffeine-sensitive and canavanine-resistant phenotypes. Based on these results, we discuss the function of Oga1 on the chronological lifespan of fission yeast.
Volume 288(5-6)
Pages 285-95
Published 2013-6
DOI 10.1007/s00438-013-0748-6
PMID 23640107
MeSH Caffeine / pharmacology Canavanine / pharmacology Cloning, Molecular DNA-Binding Proteins / genetics* DNA-Binding Proteins / metabolism Drug Resistance, Fungal / genetics G-Quadruplexes* Gene Deletion Gene Expression Regulation, Fungal Genetic Complementation Test Protein Kinases / genetics Saccharomyces cerevisiae / drug effects Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae Proteins / genetics Schizosaccharomyces / drug effects Schizosaccharomyces / physiology* Schizosaccharomyces pombe Proteins / genetics* Schizosaccharomyces pombe Proteins / metabolism
IF 2.879
Times Cited 12
WOS Category GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Yeast BY23849(BY4741)