| RRC ID |
21793
|
| Author |
Wang L, Kiuchi T, Fujii T, Daimon T, Li M, Banno Y, Katsuma S, Shimada T.
|
| Title |
Reduced expression of the dysbindin-like gene in the Bombyx mori ov mutant exhibiting mottled translucency of the larval skin.
|
| Journal |
Genome
|
| Abstract |
The ov (mottled translucent of Var) mutant, an oily mutant of Bombyx mori, exhibits mottled translucent skin with a varying degree of transparency among individuals. By linkage analysis of 2112 backcross individuals using polymorphic DNA markers, we successfully mapped a 179-kb region of chromosome 20 responsible for the ov phenotype. This region contains nine predicted genes. We compared the mRNA expression of these nine genes between the wild type and mutants and found that the expression of one of them, Bmdysb, was strikingly decreased in the epidermis of ov as well as its allelomorph, ov(p). Moreover, its expression level was well correlated with the degree of transparency among individuals. Bmdysb was homologous to DTNBP1 encoding human dysbindin, a subunit of the biogenesis of lysosome-related organelles complex-1. Our results suggest that the translucent skin may be due to repression of Bmdysb in the ov mutants and that Bmdysb plays an important role in the formation and accumulation of urate granules in the silkworm epidermis.
|
| Volume |
56(2)
|
| Pages |
101-8
|
| Published |
2013-2-1
|
| DOI |
10.1139/gen-2012-0127
|
| Description |
ov(ヴァル斑油）の原因遺伝子はBmdysbである。Bmdysbはヒトのヘルマンスキーパドラック症候群の原因遺伝子の一つであるdysbindinのカイコホモログである。o65系統(ov/ov)とo67系統(ovp/ovp)では、BmdysbのmRNAの発現量が低下しているが、低下の原因は明らかになっていない。
|
| PMID |
23517319
|
| MeSH |
Alleles
Animals
Bombyx / genetics*
Bombyx / growth & development
Carrier Proteins / genetics
Carrier Proteins / metabolism*
Chromosome Mapping
Crosses, Genetic
Epidermis / metabolism
Genes, Insect*
Genetic Linkage
Insect Proteins / genetics
Insect Proteins / metabolism*
Larva / genetics
Larva / metabolism
Mutation
Phylogeny
RNA, Messenger / metabolism
Transcription, Genetic
|
| IF |
2.037
|
| Times Cited |
14
|
|
WOS Category
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
GENETICS & HEREDITY
|
| Resource |
| Silkworms |
o66
o67
m60 |
