論文 - 詳細
| RRC ID | 21801 |
|---|---|
| 著者 | Nakashima K, Arai S, Suzuki A, Nariai Y, Urano T, Nakayama M, Ohara O, Yamamura K, Yamamoto K, Miyazaki T. |
| タイトル | PAD4 regulates proliferation of multipotent haematopoietic cells by controlling c-myc expression. |
| ジャーナル | Nat Commun |
| Abstract |
Peptidylarginine deiminase 4 (PAD4) functions as a transcriptional coregulator by catalyzing the conversion of histone H3 arginine residues to citrulline residues. Although the high level of PAD4 expression in bone marrow cells suggests its involvement in haematopoiesis, its precise contribution remains unclear. Here we show that PAD4, which is highly expressed in lineage(-) Sca-1(+) c-Kit(+) (LSK) cells of mouse bone marrow compared with other progenitor cells, controls c-myc expression by catalyzing the citrullination of histone H3 on its promoter. Furthermore, PAD4 is associated with lymphoid enhancer-binding factor 1 and histone deacetylase 1 at the upstream region of the c-myc gene. Supporting these findings, LSK cells, especially multipotent progenitors, in PAD4-deficient mice show increased proliferation in a cell-autonomous fashion compared with those in wild-type mice. Together, our results strongly suggest that PAD4 regulates the proliferation of multipotent progenitors in the bone marrow by controlling c-myc expression. |
| 巻・号 | 4 |
| ページ | 1836 |
| 公開日 | 2013-1-1 |
| DOI | 10.1038/ncomms2862 |
| PII | ncomms2862 |
| PMID | 23673621 |
| PMC | PMC3674250 |
| MeSH | Animals Cell Count Cell Nucleus / enzymology Cell Proliferation HEK293 Cells Hematopoietic Stem Cells / cytology* Hematopoietic Stem Cells / enzymology* Histone Deacetylases / metabolism Humans Hydrolases / deficiency Hydrolases / metabolism* Lymphoid Enhancer-Binding Factor 1 / metabolism Mice Mice, Inbred C57BL Multipotent Stem Cells / cytology* Multipotent Stem Cells / enzymology* Promoter Regions, Genetic / genetics Protein Transport Protein-Arginine Deiminase Type 4 Proto-Oncogene Proteins c-myc / genetics Proto-Oncogene Proteins c-myc / metabolism* |
| IF | 12.121 |
| 引用数 | 26 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC00144 |