Reference - Detail
|Author||Takegawa K, Hosomi A, Iwaki T, Fujita Y, Morita T, Tanaka N.|
|Title||Identification of a SNARE protein required for vacuolar protein transport in Schizosaccharomyces pombe.|
|Journal||Biochem. Biophys. Res. Commun.|
Intracellular vesicle trafficking is mediated by a set of SNARE proteins in eukaryotic cells. Several SNARE proteins are required for vacuolar protein transport and vacuolar biogenesis in Saccharomyces cerevisiae. A search of the Schizosaccharomyces pombe genome database revealed a total of 17 SNARE-related genes. Although no homologs of Vam3p, Nyv1p, and Vam7p have been found in S. pombe, we identified one SNARE-like protein that is homologous to S. cerevisiae Pep12p. However, the disruptants transport vacuolar hydrolase CPY (SpCPY) to the vacuole normally, suggesting that the Pep12 homolog is not required for vacuolar protein transport in S. pombe cells. To identify the SNARE protein(s) involved in Golgi-to-vacuole protein transport, we have deleted four SNARE homolog genes in S. pombe. SpCPY was significantly missorted to the cell surface on deletion of one of the SNARE proteins, Fsv1p (SPAC6F12.03c), with no apparent S. cerevisiae ortholog. In addition, sporulation, endocytosis, and in vivo vacuolar fusion appear to be normal in fsv1Delta cells. These results showed that Fsv1p is mainly involved in vesicle-mediated protein transport between the Golgi and vacuole in S. pombe cells.
|MeSH||Amino Acid Sequence Endocytosis / physiology Golgi Apparatus / chemistry Golgi Apparatus / metabolism* Membrane Fusion / physiology Membrane Proteins / chemistry* Membrane Proteins / classification Membrane Proteins / genetics Membrane Proteins / metabolism* Molecular Sequence Data Protein Transport / physiology SNARE Proteins Saccharomyces cerevisiae / chemistry Saccharomyces cerevisiae / cytology Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism Schizosaccharomyces / chemistry* Schizosaccharomyces / cytology Schizosaccharomyces / genetics Schizosaccharomyces / metabolism* Sequence Alignment* Sequence Analysis, Protein Species Specificity Vacuoles / chemistry Vacuoles / metabolism* Vesicular Transport Proteins*|
|WOS Category||BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY|