| RRC ID |
21815
|
| Author |
Pai TP, Chen CC, Lin HH, Chin AL, Lai JS, Lee PT, Tully T, Chiang AS.
|
| Title |
Drosophila ORB protein in two mushroom body output neurons is necessary for long-term memory formation.
|
| Journal |
Proc Natl Acad Sci U S A
|
| Abstract |
Memory is initially labile and gradually consolidated over time through new protein synthesis into a long-lasting stable form. Studies of odor-shock associative learning in Drosophila have established the mushroom body (MB) as a key brain structure involved in olfactory long-term memory (LTM) formation. Exactly how early neural activity encoded in thousands of MB neurons is consolidated into protein-synthesis-dependent LTM remains unclear. Here, several independent lines of evidence indicate that changes in two MB vertical lobe V3 (MB-V3) extrinsic neurons are required and contribute to an extended neural network involved in olfactory LTM: (i) inhibiting protein synthesis in MB-V3 neurons impairs LTM; (ii) MB-V3 neurons show enhanced neural activity after spaced but not massed training; (iii) MB-V3 dendrites, synapsing with hundreds of MB α/β neurons, exhibit dramatic structural plasticity after removal of olfactory inputs; (iv) neurotransmission from MB-V3 neurons is necessary for LTM retrieval; and (v) RNAi-mediated down-regulation of oo18 RNA-binding protein (involved in local regulation of protein translation) in MB-V3 neurons impairs LTM. Our results suggest a model of long-term memory formation that includes a systems-level consolidation process, wherein an early, labile olfactory memory represented by neural activity in a sparse subset of MB neurons is converted into a stable LTM through protein synthesis in dendrites of MB-V3 neurons synapsed onto MB α lobes.
|
| Volume |
110(19)
|
| Pages |
7898-903
|
| Published |
2013-5-7
|
| DOI |
10.1073/pnas.1216336110
|
| PII |
1216336110
|
| PMID |
23610406
|
| PMC |
PMC3651462
|
| MeSH |
Animals
Crosses, Genetic
Cyclic AMP Response Element-Binding Protein / metabolism
Drosophila Proteins / metabolism
Drosophila Proteins / physiology*
Drosophila melanogaster / genetics
Drosophila melanogaster / physiology*
Fragile X Mental Retardation Protein / metabolism
Gene Expression Regulation*
Memory, Long-Term / physiology*
Models, Neurological
Mushroom Bodies / metabolism
Mushroom Bodies / physiology*
Neurons / metabolism
RNA-Binding Proteins / metabolism
RNA-Binding Proteins / physiology*
Synaptic Transmission
Transcription Factors / metabolism
mRNA Cleavage and Polyadenylation Factors / metabolism
|
| IF |
9.412
|
| Times Cited |
64
|
|
WOS Category
|
NEUROSCIENCES
|
| Resource |
| Drosophila |
10868R-1
10868R-5 |
