RRC ID 2185
Author Yamada T, Iwasaki Y, Tada H, Iwabuki H, Chuah MK, VandenDriessche T, Fukuda H, Kondo A, Ueda M, Seno M, Tanizawa K, Kuroda S.
Title Nanoparticles for the delivery of genes and drugs to human hepatocytes.
Journal Nat. Biotechnol.
Abstract Hepatitis B virus envelope L particles form hollow nanoparticles displaying a peptide that is indispensable for liver-specific infection by hepatitis B virus in humans. Here we demonstrate the use of L particles for the efficient and specific transfer of a gene or drug into human hepatocytes both in culture and in a mouse xenograft model. In this model, intravenous injection of L particles carrying the gene for green fluorescent protein (GFP) or a fluorescent dye resulted in observable fluorescence only in human hepatocellular carcinomas but not in other human carcinomas or in mouse tissues. When the gene encoding human clotting factor IX was transferred into the xenograft model using L particles, factor IX was produced at levels relevant to the treatment of hemophilia B. The yeast-derived L particle is free of viral genomes, highly specific to human liver cells and able to accommodate drugs as well as genes. These advantages should facilitate targeted delivery of genes and drugs to the human liver.
Volume 21(8)
Pages 885-90
Published 2003-8
DOI 10.1038/nbt843
PII nbt843
PMID 12833071
MeSH Animals Cell Line, Tumor DNA / administration & dosage* Drug Delivery Systems / methods* Electroporation / methods Feasibility Studies Gene Targeting / methods* Genetic Therapy / methods Hepatocytes / metabolism* Humans Male Mice Microspheres Molecular Sequence Data Nanotechnology / instrumentation Nanotechnology / methods* Neoplasms / metabolism* Particle Size Transfection / methods* Viral Envelope Proteins / pharmacokinetics*
IF 31.864
Times Cited 170
Yeast BY8229(AH22R-)