RRC ID 21870
著者 Bodai L, Zsindely N, Gáspár R, Kristó I, Komonyi O, Boros IM.
タイトル Ecdysone induced gene expression is associated with acetylation of histone H3 lysine 23 in Drosophila melanogaster.
ジャーナル PLoS One
Abstract Posttranslational modification of histones regulates transcription but the exact role that acetylation of specific lysine residues plays in biological processes in vivo is still not clearly understood. To assess the contribution of different histone modifications to transcriptional activation in vivo, we determined the acetylation patterns on the ecdysone induced Eip74EF and Eip75B genes in Drosophila melanogaster larvae by chromatin immunoprecipitation. We found that acetylation of histone H3 lysine 23 is localized to promoters and correlates with endogenous ecdysone induced gene activation. In contrast, acetylation of lysines 8, 12 and 16 of histone H4 and lysine 9 of histone H3 showed minor differences in their distribution on the regulatory and transcribed regions tested, and had limited or no correlation with ecdysone induced transcriptional activity. We found that dCBP, which is encoded by the nejire gene, acetylates H3 lysine 23 in vivo, and silencing of nejire leads to reduced expression of the Eip74EF and Eip75B genes. Our results suggest that acetylation of specific lysine residues of histones contribute specifically to the dynamic regulation of transcription. Furthermore, along with previous studies identify CBP dependent H3 lysine 23 acetylation as an evolutionarily conserved chromatin modification involved in steroid induced gene activation.
巻・号 7(7)
ページ e40565
公開日 2012-7-10
DOI 10.1371/journal.pone.0040565
PII PONE-D-12-02109
PMID 22808194
PMC PMC3393682
MeSH Acetylation / drug effects Acetyltransferases / metabolism Animals DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Drosophila Proteins / genetics Drosophila Proteins / metabolism Drosophila melanogaster / drug effects* Drosophila melanogaster / genetics* Drosophila melanogaster / growth & development Ecdysone / pharmacology* Gene Expression Regulation / drug effects* Genes, Insect / genetics Histones / metabolism* Larva / genetics Larva / growth & development Lysine / metabolism* Models, Genetic Promoter Regions, Genetic / genetics Protein Processing, Post-Translational / drug effects Protein Processing, Post-Translational / genetics Time Factors Transcription Factors / genetics Transcription Factors / metabolism Transcription, Genetic / drug effects p300-CBP Transcription Factors / metabolism
IF 2.74
引用数 16
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ショウジョウバエ 15319R-2