論文 - 詳細
| RRC ID | 21874 |
|---|---|
| 著者 | Petzoldt AG, Coutelis JB, Géminard C, Spéder P, Suzanne M, Cerezo D, Noselli S. |
| タイトル | DE-Cadherin regulates unconventional Myosin ID and Myosin IC in Drosophila left-right asymmetry establishment. |
| ジャーナル | Development |
| Abstract |
In bilateria, positioning and looping of visceral organs requires proper left-right (L/R) asymmetry establishment. Recent work in Drosophila has identified a novel situs inversus gene encoding the unconventional type ID myosin (MyoID). In myoID mutant flies, the L/R axis is inverted, causing reversed looping of organs, such as the gut, spermiduct and genitalia. We have previously shown that MyoID interacts physically with β-Catenin, suggesting a role of the adherens junction in Drosophila L/R asymmetry. Here, we show that DE-Cadherin co-immunoprecipitates with MyoID and is required for MyoID L/R activity. We further demonstrate that MyoIC, a closely related unconventional type I myosin, can antagonize MyoID L/R activity by preventing its binding to adherens junction components, both in vitro and in vivo. Interestingly, DE-Cadherin inhibits MyoIC, providing a protective mechanism to MyoID function. Conditional genetic experiments indicate that DE-Cadherin, MyoIC and MyoID show temporal synchronicity for their function in L/R asymmetry. These data suggest that following MyoID recruitment by β-Catenin at the adherens junction, DE-Cadherin has a twofold effect on Drosophila L/R asymmetry by promoting MyoID activity and repressing that of MyoIC. Interestingly, the product of the vertebrate situs inversus gene inversin also physically interacts with β-Catenin, suggesting that the adherens junction might serve as a conserved platform for determinants to establish L/R asymmetry both in vertebrates and invertebrates. |
| 巻・号 | 139(10) |
| ページ | 1874-84 |
| 公開日 | 2012-5-1 |
| DOI | 10.1242/dev.047589 |
| PII | dev.047589 |
| PMID | 22491943 |
| MeSH | Animals Body Patterning / genetics Body Patterning / physiology* Cadherins / genetics Cadherins / metabolism* Drosophila Drosophila Proteins / genetics Drosophila Proteins / metabolism* Immunoprecipitation Myosin Type I / genetics Myosin Type I / metabolism Myosins / genetics Myosins / metabolism* Protein Binding beta Catenin / genetics beta Catenin / metabolism |
| IF | 5.611 |
| 引用数 | 37 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | NP1548 (DGRC#104027 or DGRC#112690) |