論文 - 詳細
| RRC ID | 21877 |
|---|---|
| 著者 | Weber U, Gault WJ, Olguin P, Serysheva E, Mlodzik M. |
| タイトル | Novel regulators of planar cell polarity: a genetic analysis in Drosophila. |
| ジャーナル | Genetics |
| Abstract |
Planar cell polarity (PCP) is a common feature of many epithelia and epithelial organs. Although progress has been made in the dissection of molecular mechanisms regulating PCP, many questions remain. Here we describe a screen to identify novel PCP regulators in Drosophila. We employed mild gain-of-function (GOF) phenotypes of two cytoplasmic Frizzled (Fz)/PCP core components, Diego (Dgo) and Prickle (Pk), and screened these against the DrosDel genome-wide deficiency collection for dominant modifiers. Positive genomic regions were rescreened and narrowed down with smaller overlapping deficiencies from the Exelixis collection and RNAi-mediated knockdown applied to individual genes. This approach isolated new regulators of PCP, which were confirmed with loss-of-function analyses displaying PCP defects in the eye and/or wing. Furthermore, knockdown of a subset was also sensitive to dgo dosage or dominantly modified a dishevelled (dsh) GOF phenotype, supporting a role in Fz/PCP-mediated polarity establishment. Among the new "PCP" genes we identified several kinases, enzymes required for lipid modification, scaffolding proteins, and genes involved in substrate modification and/or degradation. Interestingly, one of them is a member of the Meckel-Gruber syndrome factors, associated with human ciliopathies, suggesting an important role for cell polarity in nonciliated cells. |
| 巻・号 | 191(1) |
| ページ | 145-62 |
| 公開日 | 2012-5-1 |
| DOI | 10.1534/genetics.111.137190 |
| PII | genetics.111.137190 |
| PMID | 22391484 |
| PMC | PMC3338256 |
| MeSH | Animals Cell Polarity / genetics Cell Polarity / physiology* Computational Biology* Drosophila Proteins / deficiency Drosophila Proteins / genetics Drosophila Proteins / metabolism Drosophila melanogaster / cytology* Drosophila melanogaster / genetics* Drosophila melanogaster / metabolism Eye / anatomy & histology Eye / metabolism Female Gene Knockdown Techniques Genotype Humans Male Phenotype Signal Transduction Wings, Animal / anatomy & histology Wings, Animal / metabolism |
| IF | 4.015 |
| 引用数 | 8 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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