| RRC ID |
21891
|
| 著者 |
Herranz H, Hong X, Pérez L, Ferreira A, Olivieri D, Cohen SM, Milán M.
|
| タイトル |
The miRNA machinery targets Mei-P26 and regulates Myc protein levels in the Drosophila wing.
|
| ジャーナル |
EMBO J
|
| Abstract |
MicroRNAs (miRNAs) have been implicated in cell-cycle regulation and in some cases shown to have a role in tissue growth control. Depletion of miRNAs was found to have an effect on tissue growth rates in the wing primordium of Drosophila, a highly proliferative epithelium. Dicer-1 (Dcr-1) is a double-stranded RNAseIII essential for miRNA biogenesis. Adult cells lacking dcr-1, or with reduced dcr-1 activity, were smaller than normal cells and gave rise to smaller wings. dcr-1 mutant cells showed evidence of being susceptible to competition by faster growing cells in vivo and the miRNA machinery was shown to promote G(1)-S transition. We present evidence that Dcr-1 acts by regulating the TRIM-NHL protein Mei-P26, which in turn regulates dMyc protein levels. Mei-P26 is a direct target of miRNAs, including the growth-promoting bantam miRNA. Thus, regulation of tissue growth by the miRNA pathway involves a double repression mechanism to control dMyc protein levels in a highly proliferative and growing epithelium.
|
| 巻・号 |
29(10)
|
| ページ |
1688-98
|
| 公開日 |
2010-5-19
|
| DOI |
10.1038/emboj.2010.69
|
| PII |
emboj201069
|
| PMID |
20400939
|
| PMC |
PMC2876971
|
| MeSH |
Animals
Cell Cycle
Cell Proliferation
Drosophila Proteins / metabolism*
Drosophila melanogaster
E2F Transcription Factors / metabolism
Epithelium / metabolism
Female
G1 Phase
Genotype
Male
MicroRNAs / metabolism*
Models, Biological
Proto-Oncogene Proteins c-myc / metabolism*
RNA Helicases / metabolism*
Ribonuclease III / metabolism*
S Phase
Wings, Animal / metabolism
|
| IF |
9.889
|
| 引用数 |
33
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ショウジョウバエ |
12218R-1
12218R-3 |
